College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, 61 Daizong Road, Tai'an 271000, Shandong Province, China; Collaborative Innovation Centre for the Origin and Control of Emerging Infectious Diseases of Taishan Medical College, Tai'an 271000, Shandong Province, China.
Tai'an City Central Hospital, China.
Sci Total Environ. 2019 May 15;665:33-40. doi: 10.1016/j.scitotenv.2019.01.272. Epub 2019 Jan 25.
Different samples were collected from three swine farms in China to investigate the spread of antibiotic-resistant Escherichia coli. A total of 130 E. coli isolates were obtained from feces, air, river water, silt, and soil samples and characterized. The susceptibility of the E. coli isolates to 19 antibiotics was tested. The results revealed that the resistance rates of the E. coli isolates against 9 antibiotics were high. The minimum inhibitory concentration (MIC) values of ciprofloxacin, ofloxacin, and nalidixic acid were mainly in the ranges of 2-64, 8-64, and 8-64 μg/ml. The plasmid-mediated quinolone resistance (PMQR) genes qnr, aac(6')-Ib-cr, qepA, and oqxAB were detected by polymerase chain reaction (PCR), and the similarity of E. coli from different samples was identified by pulsed-field gel electrophoresis (PFGE). The detection rates of the qnrA, qnrB, qnrS, aac(6')-Ib-cr, qepA, and oqxAB genes in the E. coli isolates from three swine farms were in the range of 10.87-23.08%, 13.04-20.51%, 40.00-43.48%, 30.43-38.46%, 6.52-12.82%, and 7.69-17.39%, respectively. The PFGE result showed that 49% (49/100) of isolates originating from air, river water, soil, and silt samples had ≥85% similarity to fecal-obtained isolates, and 40.82% (20/49) of them shared the same PMQR genes with fecal-obtained isolates. This indicated that E. coli carrying PMQR genes and originating from feces in swine farms could spread to the external environment, which could be a potential threat to the public environment and human health.
从中国的三个养猪场采集了不同的样本，以调查抗生素耐药大肠杆菌的传播情况。从粪便、空气、河水、淤泥和土壤样本中获得了总共 130 株大肠杆菌分离株，并对其进行了特征描述。测试了这些大肠杆菌分离株对 19 种抗生素的敏感性。结果表明，这些大肠杆菌分离株对 9 种抗生素的耐药率很高。环丙沙星、氧氟沙星和萘啶酸的最低抑菌浓度（MIC）值主要在 2-64、8-64 和 8-64μg/ml 范围内。通过聚合酶链反应（PCR）检测了质粒介导的喹诺酮耐药（PMQR）基因 qnr、aac(6')-Ib-cr、qepA 和 oqxAB，并通过脉冲场凝胶电泳（PFGE）鉴定了来自不同样本的大肠杆菌的相似性。在来自三个养猪场的大肠杆菌分离株中，qnrA、qnrB、qnrS、aac(6')-Ib-cr、qepA 和 oqxAB 基因的检出率分别为 10.87-23.08%、13.04-20.51%、40.00-43.48%、30.43-38.46%、6.52-12.82%和 7.69-17.39%。PFGE 结果显示，来自空气、河水、土壤和淤泥样本的 49%（49/100）的分离株与粪便中获得的分离株具有≥85%的相似性，其中 40.82%（49/100）的分离株与粪便中获得的分离株具有相同的 PMQR 基因。这表明携带 PMQR 基因的大肠杆菌源自养猪场的粪便，可以传播到外部环境，这可能对公共环境和人类健康构成潜在威胁。
