Department of Obstetrics and Gynecology, Keio University Graduate School of Medicine, 35 Shinanomachi, -Shinjyuku-ku, Tokyo 160-8582, Japan; Reproduction and Infertility Centre, Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan.
Reproduction and Infertility Centre, Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan; Advanced Reproductive Medicine Research Centre, International University Health and Welfare School of Medicine, 4-3 Kozunomori, Narita Shi, Chiba 286-8686, Japan.
Reprod Biomed Online. 2019 Apr;38(4):570-578. doi: 10.1016/j.rbmo.2018.12.026. Epub 2018 Dec 22.
Do gut microbiota associate with the ovulatory cycle in women showing normogonadotrophic anovulation? In humans, the gut microbiota affects diverse physiological functions and dysbiosis (microbial imbalance) may lead to pathological syndromes. However, there is comparatively little information on the relevance of gut microbiota to reproductive functions in women. Here, a group of women with idiopathic chronic anovulation were examined, who do not exhibit any apparent endocrinological disorder, as they are suitable for investigating the relationship between intestinal bacteria and ovulatory disorders.
A prospective observational cohort study was performed on two groups of women who did not exhibit apparent endocrinological disorders but showed either irregular menstrual cycles (IMC group) or normal menstrual cycles (controls). The bacterial composition of faeces from rectal swabs from the women was analysed using next-generation sequencing based on bacterial 16SrRNA genes.
A metagenomic analysis indicated that the two groups of women had significant differences in 28 bacterial taxa in their faeces. Prevotella-enriched microbiomes were more abundant in the IMC group, whereas Clostridiales, Ruminococcus and Lachnospiraceae (butyrate-producing bacteria) were present at lower levels in the IMC group.
Distinctive subpopulations of intestinal microbiota were identified in women with unexplained chronic anovulation. The results indicate that gut microbiota could be associated with ovarian functions.
在表现为正常促性腺激素性无排卵的女性中，肠道微生物群是否与排卵周期有关？在人类中，肠道微生物群影响多种生理功能，而微生物失调（微生物失衡）可能导致病理综合征。然而，关于肠道微生物群与女性生殖功能的相关性的信息相对较少。在这里，研究了一组患有特发性慢性无排卵的女性，她们没有表现出任何明显的内分泌紊乱，因为她们适合研究肠道细菌与排卵障碍之间的关系。
对两组未表现出明显内分泌紊乱但表现出不规则月经周期（IMC 组）或正常月经周期（对照组）的女性进行了前瞻性观察队列研究。使用基于细菌 16SrRNA 基因的下一代测序分析来自直肠拭子的粪便中的细菌组成。
宏基因组分析表明，两组女性粪便中的 28 种细菌分类群存在显著差异。IMC 组中普雷沃氏菌富集的微生物组更为丰富，而 IMC 组中梭状芽胞杆菌、瘤胃球菌和lachnospiraceae（产生丁酸的细菌）的水平较低。
在不明原因的慢性无排卵女性中鉴定出了独特的肠道微生物亚群。结果表明，肠道微生物群可能与卵巢功能有关。
