Yin Cui-Cui, Ma Biao, Wang Wei, Xiong Qing, Zhao He, Chen Shou-Yi, Zhang Jin-Song
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Curr Protoc Plant Biol. 2016 Mar;1(2):411-418. doi: 10.1002/cppb.20023.
There has been a surge of interest in the molecular biology of RNA. We describe a simple, fast, and convenient protocol for isolating total RNA from rice (Oryza sativa) seedlings and young tissues/organs. RNA obtained using this method can be used for reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR (qRT-PCR), northern blot analysis, RNA-seq, microarray analysis, cDNA library construction, etc. Technical parameters critical to the success of the extraction are presented. © 2016 by John Wiley & Sons, Inc.
人们对RNA分子生物学的兴趣激增。我们描述了一种从水稻（Oryza sativa）幼苗和幼嫩组织/器官中分离总RNA的简单、快速且便捷的方法。使用该方法获得的RNA可用于逆转录聚合酶链反应（RT-PCR）、定量RT-PCR（qRT-PCR）、Northern印迹分析、RNA测序、微阵列分析、cDNA文库构建等。文中还介绍了对提取成功至关重要的技术参数。© 2016约翰威立国际出版公司。
