He Feng, Chen Songbiao, Ning Yuese, Wang Guo-Liang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.
Curr Protoc Plant Biol. 2016 Mar;1(2):373-383. doi: 10.1002/cppb.20026.
Rice (Oryza sativa) is not only the staple food for half of the world's population but also a model monocot plant for molecular biology studies. Although rice genes have been extensively investigated in the last two decades, the functions of many genes in the rice genome are still not known. One of the rapid and efficient approaches for determining gene function in vivo is protoplast-based transient expression analysis. We established a rice protoplast system about 10 years ago, which has been recently used in many laboratories. This protocol is useful for protein expression, subcellular localization, bimolecular fluorescence complementation, and co-immunoprecipitation assays. © 2016 by John Wiley & Sons, Inc.
水稻（Oryza sativa）不仅是世界上一半人口的主食，也是分子生物学研究的单子叶模式植物。尽管在过去二十年里对水稻基因进行了广泛研究，但水稻基因组中许多基因的功能仍然未知。基于原生质体的瞬时表达分析是在体内确定基因功能的快速有效方法之一。大约10年前我们建立了一个水稻原生质体系统，最近该系统已在许多实验室中使用。该方案可用于蛋白质表达、亚细胞定位、双分子荧光互补和免疫共沉淀分析。© 2016约翰威立国际出版公司
