Interactions between Microcystis aeruginosa and coexisting bisphenol A at different nitrogen levels.

Microcystis aeruginosa is known as a main contributor of cyanobacterial bloom. However, factors that drive its formation and dispersion remain poorly understood. The cellular-level responses to nutrient drivers of eutrophication were investigated. The results showed that growth rate of M. aeruginosa was significantly enhanced with the increasing bisphenol A (BPA) and nitrogen (N) level. Stress of BPA significantly inhibited cellular density, chlorophyll-a content across all the nutrient conditions, while Fv/Fm and rETRmax value were promoted by BPA. Responses of reactive oxygen species (ROS) value, superoxide dismutase (SOD) activity and malodialdehyde (MDA) content indicated that nitrogen deficiency and BPA caused oxidative stress to M. aeruginosa. Besides, nitrogen and BPA regulated the production and release of microcystins (MCs). M. aeruginosa exposed to BPA caused 95 up-regulated proteins, which was primarily associated with photosynthesis, nitrogen metabolism, glycolysis/glyconeogenesis and carbon fixation in photosynthetic organisms. The 91 down-regulated proteins were related to quorum sensing, longevity regulating and cell cycle-caulobacter, confirming that the driving force of regulating the change of cellular density and genes expression weakened. These findings provide important clues to elucidate the combined regulatory mechanisms of cyanobacterial blooms triggered by endocrine-disrupting compounds and environmental factors and help to effectively prevent and reduce cyanobacterial blooms.