Yang Meng, Fan Zhengqiu, Xie Yujing, Fang Lei, Wang Xiangrong, Yuan Yuan, Li Rongxi
Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China.
Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China.
J Hazard Mater. 2020 Oct 5;397:122746. doi: 10.1016/j.jhazmat.2020.122746. Epub 2020 Apr 29.
Bisphenol A (BPA), one of the most abundant endocrine-disrupting compounds, is frequently detected in diverse aquatic environments, which imposes a substantial burden on the aquatic ecosystem. However, the correlation between BPA levels and the outbreak of a cyanobacterial bloom remains largely unknown. In this study, the cellular and transcriptomic responses to BPA exposure were investigated. Exposure to a high concentration of BPA (50 μM) significantly inhibited the growth of cyanobacterial cells, with the highest inhibition ratio of 51.3%, photosynthesis, and the release of extracellular microcystin-LR (MC-LR) (p < 0.05). However, exposure to low concentrations of BPA (0.1 and 1 μM) also affected these indicators, but the differences were closely related to the growth phase of the cyanobacterial cells. In addition, an imbalance between the antioxidant system and oxidative stress was observed in cyanobacteria under BPA stress. Folate biosynthesis, ABC transporters and ubiquinone and other terpenoid-quinone biosynthesis were the central metabolic pathways triggered by BPA stress. The up-regulated genes, including queC, VTE3 and PsbO were the controller of cellular growth and photosynthesis. The down-regulated genes, including VET4, MlaE and DnaA were potential biomarkers of oxidative damage. The up- and down-regulated genes, including CA, Ppc and CyoE were the main regulators of energy generation. The findings will provide important insights into the role of endocrine disruptors in the frequent outbreak of cyanobacterial blooms.
双酚A（BPA）是最常见的内分泌干扰化合物之一，在各种水生环境中经常被检测到，这给水生生态系统带来了巨大负担。然而，BPA水平与蓝藻水华爆发之间的相关性在很大程度上仍不清楚。在本研究中，研究了蓝藻对BPA暴露的细胞和转录组反应。暴露于高浓度BPA（50μM）显著抑制了蓝藻细胞的生长，最高抑制率为51.3%，同时抑制了光合作用和细胞外微囊藻毒素-LR（MC-LR）的释放（p<0.05）。然而，暴露于低浓度BPA（0.1和1μM）也会影响这些指标，但差异与蓝藻细胞的生长阶段密切相关。此外，在BPA胁迫下，蓝藻中观察到抗氧化系统与氧化应激之间的失衡。叶酸生物合成、ABC转运蛋白以及泛醌和其他萜类醌生物合成是BPA胁迫触发的中心代谢途径。上调基因，包括queC、VTE3和PsbO，是细胞生长和光合作用的调控因子。下调基因，包括VET4、MlaE和DnaA，是氧化损伤的潜在生物标志物。上调和下调基因，包括CA、Ppc和CyoE，是能量产生的主要调节因子。这些发现将为内分泌干扰物在蓝藻水华频繁爆发中的作用提供重要见解。
