Department of Science of Biological Production, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan.
Department of United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan.
Sci Rep. 2019 Feb 18;9(1):2170. doi: 10.1038/s41598-018-38384-8.
Insects are well adapted to changing environmental conditions. They have unique systems for eliminating reactive oxygen species (ROS). Superoxide dismutase (SOD) is a key enzyme that plays a primary role in removing ROS. Bombyx mori is a lepidopteran insect, whose body size is larger than the model insect Drosophila melanogaster, which enabled us to more easily examine gene expression at the tissue level. We searched B. mori SOD (BmSOD) genes using genome database, and we analyzed their function under different type of oxidative stress. Consequently, we identified four new types of BmSODs in addition to the three types already known. Two of the seven types had a unique domain architecture that has not been discovered previously in the SOD family, and they were expressed in different tissues and developmental stages. Furthermore, these BmSODs responded differently to several kinds of stressors. Our results showed that the seven types of BmSODs are likely to play different roles in B. mori; therefore, B. mori could be used to distinguish the functions of each SOD for resistance to oxidative stress that changes with the environmental conditions.
昆虫很好地适应了环境变化。它们具有独特的清除活性氧（ROS）的系统。超氧化物歧化酶（SOD）是一种关键酶，在清除 ROS 方面发挥着主要作用。家蚕是鳞翅目昆虫，其体型比模式昆虫黑腹果蝇大，这使我们能够更轻松地在组织水平上检查基因表达。我们使用基因组数据库搜索家蚕 SOD（BmSOD）基因，并分析它们在不同类型的氧化应激下的功能。结果，除了已经知道的三种类型外，我们还鉴定出了四种新的 BmSOD 类型。这七种类型中的两种具有独特的结构域架构，以前在 SOD 家族中尚未发现，它们在不同的组织和发育阶段表达。此外，这些 BmSOD 对几种应激原的反应不同。我们的结果表明，这七种类型的 BmSOD 可能在家蚕中发挥不同的作用；因此，家蚕可以用来区分每个 SOD 对环境条件变化的抗氧化应激的功能。
