Sericultural Research Institute,Sichuan Academy of Agricultural Sciences, Nanchong, Sichuan, China.
College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan, China.
PeerJ. 2023 Jan 13;11:e14682. doi: 10.7717/peerj.14682. eCollection 2023.
The silkworm () is not only an excellent model species, but also an important agricultural economic insect. Taking it as the research object, its advantages of low maintenance cost and no biohazard risks are considered. Small open reading frames (smORFs) are an important class of genomic elements that can produce bioactive peptides. However, the smORFs in silkworm had been poorly identified and studied. To further study the smORFs in silkworm, systematic genome-wide identification is essential. Here, we identified and analyzed smORFs in the silkworm using comprehensive methods. Our results showed that at least 738 highly reliable smORFs were found in and that 34,401 possible smORFs were partially supported. We also identified some differentially expressed and tissue-specific-expressed smORFs, which may be closely related to the characteristics and functions of the tissues. This article provides a basis for subsequent research on smORFs in silkworm, and also hopes to provide a reference point for future research methods for smORFs in other species.
家蚕（）不仅是一种优秀的模式生物，也是一种重要的农业经济昆虫。以家蚕为研究对象，其维护成本低、无生物危害风险等优点得到了考虑。小开放阅读框（smORFs）是一类能够产生生物活性肽的重要基因组元件。然而，家蚕中的 smORFs 一直未得到很好的鉴定和研究。为了进一步研究家蚕中的 smORFs，系统的全基因组鉴定是必不可少的。在这里，我们使用综合方法对家蚕中的 smORFs 进行了鉴定和分析。我们的结果表明，至少在 中发现了 738 个高度可靠的 smORFs，并且部分支持了 34401 个可能的 smORFs。我们还鉴定了一些差异表达和组织特异性表达的 smORFs，它们可能与组织的特征和功能密切相关。本文为后续研究家蚕中的 smORFs 提供了基础，也希望为其他物种 smORFs 的未来研究方法提供参考点。
