Mohammadi Reza, Mohammadi Zahra, Abedi Reza, Chireh Fatemeh, Balali Abdolreza, Mohamadynejad Parisa, Shirian Sadegh, Moghanibashi Mehdi
Department of Biology, Faculy of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Imam Ali Hospital, Farokhshar, Shahrekord, Iran.
Mol Biol Rep. 2019 Apr;46(2):2355-2362. doi: 10.1007/s11033-019-04692-z. Epub 2019 Feb 18.
A large proportion of the transcriptome is sex biased in a wide range of taxa. Sexually dimorphic genes expression is highly tissue-dependent. Although gastric cancer exhibits sex bias to some extent, sexually dimorphic gene expression in the stomach is yet to be fully understood. The aim of the present study was to compare the expression levels of 12 genes in the gastric tissue between age-matched healthy men and women of different age groups. A total of 70 human antrum gastric tissue samples were obtained by endoscopy. The difference in expression levels of the 12 intended genes between two genders was investigated using quantitative Real-Time PCR, following total RNA extraction. The results indicated that the expression levels of both the GKN2 (7.2-fold, p < 0.001) and FOXA2 (3.7-fold, p = 0.003) were significantly higher in men compared to those in women. In addition, FOXA1 gene expression was age-dependent only in women. Interestingly, the expression level of FOXA1 was significantly higher in premenopausal women compared to postmenopausal women (2.53-fold, p = 0.016). The expression levels of some of the investigated genes in this study were sex-dependent in the stomach. This sexual dimorphism in gene expression might influence the differential susceptibility to the gastric cancer between the sexes.
在广泛的生物分类群中，很大一部分转录组存在性别偏向性。性二态性基因表达具有高度的组织依赖性。尽管胃癌在一定程度上表现出性别偏向性，但胃中的性二态性基因表达尚未得到充分了解。本研究的目的是比较不同年龄组中年龄匹配的健康男性和女性胃组织中12个基因的表达水平。通过内窥镜检查共获取了70份人类胃窦组织样本。在提取总RNA后，使用定量实时PCR研究了这12个目标基因在两种性别之间表达水平的差异。结果表明，与女性相比，男性中GKN2（7.2倍，p < 0.001）和FOXA2（3.7倍，p = 0.003）的表达水平均显著更高。此外，FOXA1基因表达仅在女性中具有年龄依赖性。有趣的是，绝经前女性中FOXA1的表达水平显著高于绝经后女性（2.53倍，p = 0.016）。本研究中一些被研究基因的表达水平在胃中存在性别依赖性。这种基因表达的性二态性可能会影响两性之间对胃癌的易感性差异。
