Department of General surgery, Affiliated Haian Hospital of Nantong University, Haian County of Nantong City, China.
Eur Rev Med Pharmacol Sci. 2019 Feb;23(3):1145-1150. doi: 10.26355/eurrev_201902_17005.
MicroRNAs (miRNAs) are recently identified as key regulators of tumor development and progression. MiR-202-3p functions as tumor suppressor in some cancer types. The aim of the study is to determine its expression pattern and explore the functions underlying the mechanism of miR-202-3p in papillary thyroid carcinoma (PTC).
By using quantitative RT-PCR (QRT-PCR) analyses, we detected miR-202-3p expression in PTC tissues and cell lines. Transwell migration and invasion assays were performed to measure the migration and invasion ability of tumor cells transfected with miR-202-3p mimic. Western blot analysis was used to detect the protein expression.
Our results showed that miR-202-3p expression was frequently downregulated in 96 cases PTC tissues compared to adjacent normal tissues. Lower expression of miR-202-3p associated with lymph node metastasis of patients with PTC. Overexpression of miR-202-3p inhibited cell migration and invasion in TPC-1 and BCPAP cells. Furthermore, enforced expression of miR-202-3p inhibited WNT signaling by downregulating β-catenin expression in TPC-1 and BCPAP cells.
Our findings indicated that miR-202-3p may represent a novel therapeutic target of in PTC.
微小 RNA（miRNAs）最近被鉴定为肿瘤发生和发展的关键调节因子。miR-202-3p 在某些癌症类型中作为肿瘤抑制因子发挥作用。本研究旨在确定其表达模式，并探讨 miR-202-3p 在甲状腺乳头状癌（PTC）中的作用机制。
通过使用定量 RT-PCR（QRT-PCR）分析，我们检测了 PTC 组织和细胞系中 miR-202-3p 的表达。通过 Transwell 迁移和侵袭实验测量转染 miR-202-3p 模拟物的肿瘤细胞的迁移和侵袭能力。使用 Western blot 分析检测蛋白表达。
我们的结果表明，与相邻正常组织相比，96 例 PTC 组织中 miR-202-3p 的表达经常下调。miR-202-3p 表达较低与 PTC 患者的淋巴结转移有关。miR-202-3p 的过表达抑制了 TPC-1 和 BCPAP 细胞的迁移和侵袭。此外，在 TPC-1 和 BCPAP 细胞中，强制表达 miR-202-3p 通过下调β-catenin 表达抑制了 WNT 信号通路。
我们的研究结果表明，miR-202-3p 可能是 PTC 的一种新的治疗靶点。
