Second Military Medical University, Shanghai, China.
Institute for Laboratory Medicine, The 900th Hospital of Joint Service Support Force, Fuzhou, Fujian, China.
PLoS One. 2019 Feb 19;14(2):e0212041. doi: 10.1371/journal.pone.0212041. eCollection 2019.
This study aimed to screen DNA aptamers against the signal molecule C4-HSL of the rhl system for the inhibition of biofilm formation of Pseudomonas aeruginosa using an improved systematic evolution of ligand by exponential enrichment (SELEX) method based on a structure-switching fluorescent activating bead. The aptamers against the C4-HSL with a high affinity and specifity were successfully obtained and evaluated in real-time by this method. Results of biofilm inhibition experiments in vitro showed that the biofilm formation of P. aeruginosa was efficiently reduced to about 1/3 by the aptamers compared with that of the groups without the aptamers. Independent secondary structure simulation and computer-aided tertiary structure prediction (3dRNA) showed that the aptamers contained a highly conserved Y-shaped structural unit. Therefore, this study benefits the search for new methods for the detection and treatment of P. aeruginosa biofilm formation.
本研究旨在利用基于结构切换荧光激活珠的改进指数富集配体系统进化（SELEX）方法，筛选针对 rhl 系统信号分子 C4-HSL 的 DNA 适体，以抑制铜绿假单胞菌生物膜的形成。该方法成功获得了与 C4-HSL 具有高亲和力和特异性的适体，并进行了实时评估。体外生物膜抑制实验结果表明，与无适体组相比，适体可将铜绿假单胞菌的生物膜形成有效降低至约 1/3。独立的二级结构模拟和计算机辅助三级结构预测（3dRNA）表明，适体含有高度保守的 Y 形结构单元。因此，本研究有助于寻找新的方法来检测和治疗铜绿假单胞菌生物膜的形成。
