细胞形状和基质硬度决定基于肌动蛋白的细胞极性。
Cell shape and substrate stiffness drive actin-based cell polarity.
机构信息
Mechanobiology Institute, National University of Singapore, 117411 Singapore, Singapore.
Institut Jacques Monod, CNRS, UMR No. 7592, Université Paris Diderot, 75013 Paris, France.
出版信息
Phys Rev E. 2019 Jan;99(1-1):012412. doi: 10.1103/PhysRevE.99.012412.
Abstract
A general trait of living cells is their ability to exert contractile stresses on their surroundings and thus respond to substrate rigidity. At the cellular scale, this response affects cell shape, polarity, and ultimately migration. The regulation of cell shape together with rigidity sensing remains largely unknown. In this article we show that both substrate rigidity and cell shape contribute to drive actin organization and cell polarity. Increasing substrate rigidity affects bulk properties of the actin cytoskeleton by favoring long-lived actin stress fibers with increased nematic interactions, whereas cell shape imposes a local alignment of actin fibers at the cell periphery.
摘要
活细胞的一个普遍特征是它们能够对周围环境施加收缩力，从而对基质刚性做出反应。在细胞尺度上，这种反应会影响细胞的形状、极性，并最终影响细胞的迁移。细胞形状的调节和刚性感知在很大程度上仍然未知。在本文中，我们表明，基质刚性和细胞形状都有助于驱动肌动蛋白组织和细胞极性。增加基质刚性会通过有利于具有增强的向列相互作用的长寿命肌动蛋白应力纤维来影响肌动蛋白细胞骨架的整体性质，而细胞形状则在细胞边缘处对肌动蛋白纤维进行局部排列。
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