利用环境友好型壳聚糖纳米粒子和近红外激光实现高效癌症成像和治疗。
Toward Highly Efficient Cancer Imaging and Therapy Using the Environment-Friendly Chitosan Nanoparticles and NIR Laser.
机构信息
System and Biomedical Engineering Department, The Higher Institute of Engineering, El Shorouk City, Cairo 11837, Egypt.
Department of Laser Applications in Metrology, Photochemistry and Agriculture (LAMPA), National Institute of Laser Enhanced Sciences (NILE), Cairo University, Giza 12613, Egypt.
出版信息
Biosensors (Basel). 2019 Feb 18;9(1):28. doi: 10.3390/bios9010028.
Chitosan-tripolyphosphate nanoparticles (C-TPP NPs) were synthesized to investigate their cytotoxicity against colon cancer cells (Caco2 cells) in the absence and the presence of a near-infrared (NIR) laser to evaluate their influence in cancer detection using the NIR laser and to evaluate the NIR laser on cancer treatment. The synthesized NPs were characterized by Fourier transform infrared (FT-IR) spectroscopy, dynamic light scattering (DLS), zeta potential (ZP), and transmission electronic microscope (TEM). The cytotoxicity was analyzed by the MTT test and the cell viability was assessed using the Trypan blue method. C-TPP NPs showed increased cytotoxicity and decreased cell viability against Caco2 cells. Upon laser exposure only, the cell viability decreased. The C-TPP NPs appeared to have a shining light on the cancerous cells which were photographed under the inverted microscope.
壳聚糖-三聚磷酸钠纳米粒子（C-TPP NPs）被合成出来，以研究它们在没有和存在近红外（NIR）激光的情况下对结肠癌细胞（Caco2 细胞）的细胞毒性，以评估它们在使用 NIR 激光进行癌症检测方面的影响，并评估 NIR 激光在癌症治疗中的应用。合成的 NPs 通过傅里叶变换红外（FT-IR）光谱、动态光散射（DLS）、Zeta 电位（ZP）和透射电子显微镜（TEM）进行表征。通过 MTT 试验分析细胞毒性，通过台盼蓝法评估细胞活力。C-TPP NPs 对 Caco2 细胞表现出增强的细胞毒性和降低的细胞活力。仅在激光照射下，细胞活力下降。在倒置显微镜下拍摄的癌细胞上，C-TPP NPs 似乎发出了闪亮的光。
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