Applied Physical Chemistry, Faculty of Science, Al-Azhar University (Girls), Cairo, Egypt; Al-Azhar Technology Incubator (ATI), Cairo, Egypt.
Cancer Cell Biology, Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
Int J Biol Macromol. 2019 Apr 1;126:478-487. doi: 10.1016/j.ijbiomac.2018.12.151. Epub 2018 Dec 17.
Actually, the most common cancer in women is the breast cancer which is the second most widespread cancer overall. In 2018, there were over two million new cases of women breast cancer. Particularly, we tried to extract chitosan from crayfish Procambarus clarkii, Crustacea: Cambaridae, by N-deacetylation of chitin. The chemical structure of chitosan was characterized by Fourier transform infrared (FT-IR) spectroscopy. Also DDA was calculated from FT-IR and ultraviolet spectrophotometry data. Chitosan nanoparticles were prepared using a ball-milling technique. The as-prepared chitosan nanoparticles were characterized by transmission electron microscopy, dynamic light scattering as well as zeta potential. The cytotoxicity of chitosan and its nanoparticles (50 and 100 μg/mL) against human breast cancer (SK BR3 and MDA-MB-231 cell lines) was evaluated. MTT assay asserts the significant inhibitory action of both chitosan and its nanoparticles on the proliferation of human breast cancer cells in vitro. Chitosan nanoparticles had more anti-proliferative effects on MDA-MB-231 and SK-BR-3 cell lines than its corresponding chitosan. Although, chitosan nanoparticles, that has higher DDA, had a higher cytotoxic activity against human breast cancer MDA-MB-231 and SK-BR-3 cell lines in vitro. Eventually, chitosan and its nanoparticles can be considered as a promising natural compounds in human breast cancer treatment.
实际上，女性最常见的癌症是乳腺癌，它是总体上第二大常见的癌症。2018 年，有超过 200 万例新的女性乳腺癌病例。特别是，我们试图通过几丁质的 N-脱乙酰化从淡水小龙虾 Procambarus clarkii（十足目：长臂虾科）中提取壳聚糖。壳聚糖的化学结构通过傅里叶变换红外（FT-IR）光谱进行了表征。此外，根据 FT-IR 和紫外分光光度法数据计算了 DDA。使用球磨技术制备壳聚糖纳米粒子。通过透射电子显微镜、动态光散射以及zeta 电位对所制备的壳聚糖纳米粒子进行了表征。评估了壳聚糖及其纳米粒子（50 和 100μg/mL）对人乳腺癌（SK BR3 和 MDA-MB-231 细胞系）的细胞毒性。MTT 测定法证实了壳聚糖及其纳米粒子对体外人乳腺癌细胞增殖的显著抑制作用。壳聚糖纳米粒子对 MDA-MB-231 和 SK-BR-3 细胞系的增殖具有更强的抗增殖作用，其效果优于相应的壳聚糖。尽管具有更高 DDA 的壳聚糖纳米粒子对体外人乳腺癌 MDA-MB-231 和 SK-BR-3 细胞系具有更高的细胞毒性活性。最终，壳聚糖及其纳米粒子可以被认为是治疗人类乳腺癌的有前途的天然化合物。
