Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032-8202, USA.
Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032-8202, USA.
Anal Chim Acta. 2019 May 9;1055:74-80. doi: 10.1016/j.aca.2019.01.009. Epub 2019 Jan 18.
This paper describes the development of two microfluidic paper-based analytical devices (μPADs), one well-based and the other based on a lateral flow assay (LFA) configuration, to detect glucose via a colorimetric assay using the solid metal-organic framework (MOF) Zr-PCN-222(Fe), to encapsulate glucose oxidase (GOx). The well-based platform consisted of laminate sheets and multiple layers of wax-printed chromatography paper. Solutions of KI and glucose placed into the well flowed through the device and reacted with the GOx@MOF species sandwiched between the paper layers realizing a yellow-brown color. The LFA platform consisted of chromatography paper between parafilm and polyvinyl acetate (PVA) layers. GOx@MOFs spotted on the paper subjected to solutions of KI and glucose yielded a brown color. The devices were then dried, scanned, and analyzed yielding a correlation between average inverse yellow intensity and glucose concentrations. The development of these devices employing MOFs as biomimetic catalysts should further expand the applications of microfluidic technologies for sensors a variety of analytes.
本文描述了两种基于微流控纸的分析装置（μPAD）的开发，一种是基于孔的，另一种是基于侧流分析（LFA）配置的，通过使用固态金属-有机骨架（MOF）Zr-PCN-222（Fe）进行比色分析来检测葡萄糖，以封装葡萄糖氧化酶（GOx）。基于孔的平台由层压片材和多层蜡印色谱纸组成。将 KI 和葡萄糖溶液放入孔中，使其流过装置，并与夹在纸层之间的 GOx@MOF 物种反应，实现黄色-棕色的颜色。LFA 平台由中间夹有 Parafilm 和聚醋酸乙烯酯（PVA）层的色谱纸组成。GOx@MOFs 点样在纸上，然后用 KI 和葡萄糖溶液处理，产生棕色颜色。然后将这些设备干燥、扫描并进行分析，得到平均反向黄色强度与葡萄糖浓度之间的相关性。这些使用 MOF 作为仿生催化剂的设备的开发应进一步扩展微流控技术在各种分析物传感器中的应用。
