Division of Biotechnology, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
University of Chinese Academy of Sciences, Beijing, China.
J Biomed Mater Res B Appl Biomater. 2019 Nov;107(8):2527-2536. doi: 10.1002/jbm.b.34343. Epub 2019 Feb 19.
Sodium alginate (NaA) has been widely used as microfiber-based scaffold material. However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering. In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device. A more stable composite microfiber with double cross-linking layers was generated. The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure. The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution. In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber. HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability. We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019.
海藻酸钠（NaA）已被广泛用作基于微纤维的支架材料。然而，由于钙离子的流失，海藻酸钙微纤维可能会在生理环境中解体，这将限制其在组织工程中的长期应用。在这项工作中，为了提高 Ca-海藻酸钠微纤维在生理环境中的稳定性，通过微流控装置一步法将壳聚糖内层涂层引入 Ca-海藻酸钠中空微纤维中。生成了具有双层交联层的更稳定的复合微纤维。在 PBS 溶液（pH7.4）中研究了微纤维的稳定性，以确定涂层对中空结构的影响。结果表明，壳聚糖组分在微纤维的内壁上结合了一层 NaA，形成了稳定的聚电解质复合膜，即使 Ca-海藻酸钠壳被 PBS 溶液破坏，也能稳定中空区域。此外，壳聚糖涂层的引入改善了海藻酸钠对细胞表面低亲和力的内部环境，有利于细胞在微纤维中的粘附和培养。微纤维中的 HepG2 细胞表现出良好的细胞活力和增殖能力。我们相信，这项工作将为细胞培养和组织工程应用的创新方法和材料的发展奠定基础。©2019 年 Wiley 期刊，生物医学高分子杂志 B 部分：应用生物材料 107B：2527-2536，2019。
