Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, P.O. Box: 143951374, Tehran, Iran.
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, P.O. Box: 143951374, Tehran, Iran.
Carbohydr Polym. 2016 Oct 20;151:1120-1131. doi: 10.1016/j.carbpol.2016.06.063. Epub 2016 Jun 21.
The chemical nature of biomaterials play important role in cell attachment, proliferation and migration in tissue engineering. Chitosan and alginate are biodegradable and biocompatible polymers used as scaffolds for various medical and clinical applications. Amine groups of chitosan scaffolds play an important role in cell attachment and water adsorption but also associate with alginate carboxyl groups via electrostatic interactions and hydrogen bonding, consequently the activity of amine groups in the scaffold decreases. In this study, chitosan/alginate/halloysite nanotube (HNTs) composite scaffolds were prepared using a freeze-drying method. Amine treatment on the scaffold occurred through chemical methods, which in turn caused the hydroxyl groups to be replaced with carboxyl groups in chitosan and alginate, after which a reaction between ethylenediamine, 1-ethyl-3,(3-dimethylaminopropyl) carbodiimide (EDC) and scaffold triggered the amine groups to connect to the carboxyl groups of chitosan and alginate. The chemical structure, morphology and mechanical properties of the composite scaffolds were investigated by FTIR, CHNS, SEM/EDS and compression tests. The electrostatic attraction and hydrogen bonding between chitosan, alginate and halloysite was confirmed by FTIR spectroscopy. Chitosan/alginate/halloysite scaffolds exhibit significant enhancement in compressive strength compared with chitosan/alginate scaffolds. CHNS and EDS perfectly illustrate that amine groups were effectively introduced in the aminated scaffold. The growth and cell attachment of L929 cells as well as the cytotoxicity of the scaffolds were investigated by SEM and Alamar Blue (AB). The results indicated that the aminated chitosan/alginate/halloysite scaffold has better cell growth and cell adherence in comparison to that of chitosan/alginate/halloysite samples. Aminated chitosan/alginate/halloysite composite scaffolds exhibit great potential for applications in tissue engineering, ideally in cell culture.
生物材料的化学性质在组织工程中的细胞附着、增殖和迁移中起着重要作用。壳聚糖和海藻酸盐是可生物降解和生物相容的聚合物，可用作各种医疗和临床应用的支架。壳聚糖支架的胺基在细胞附着和水吸附中起着重要作用，但也通过静电相互作用和氢键与海藻酸盐的羧基结合，因此支架中胺基的活性降低。在这项研究中，使用冷冻干燥法制备了壳聚糖/海藻酸盐/海泡石纳米管（HNTs）复合支架。支架上的胺处理通过化学方法进行，这反过来导致壳聚糖和海藻酸盐中的羟基被羧基取代，之后乙二胺、1-乙基-3，（3-二甲基氨基丙基）碳二亚胺（EDC）与支架之间的反应触发胺基与壳聚糖和海藻酸盐的羧基连接。通过傅里叶变换红外光谱（FTIR）、CHNS、扫描电子显微镜/能谱（SEM/EDS）和压缩试验研究了复合支架的化学结构、形态和机械性能。FTIR 光谱证实了壳聚糖、海藻酸盐和海泡石之间的静电吸引和氢键。壳聚糖/海藻酸盐/海泡石支架的抗压强度与壳聚糖/海藻酸盐支架相比有显著提高。CHNS 和 EDS 完全说明了胺基有效地引入了氨基支架中。通过 SEM 和 Alamar Blue（AB）研究了 L929 细胞的生长和细胞附着以及支架的细胞毒性。结果表明，与壳聚糖/海藻酸盐/海泡石样品相比，氨基化壳聚糖/海藻酸盐/海泡石支架具有更好的细胞生长和细胞粘附性。氨基化壳聚糖/海藻酸盐/海泡石复合支架在组织工程应用中具有很大的潜力，尤其是在细胞培养方面。
