College of Land Science and Technology, China University of Geosciences (Beijing), Beijing 100083, China.; State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
J Environ Sci (China). 2019 May;79:321-329. doi: 10.1016/j.jes.2018.12.007. Epub 2018 Dec 24.
Calcium carbonate (CaCO) application is widely used to ameliorate soil acidification. To counteract soil and bacterial community response to CaCO application in an acidic paddy soil in southern China, a field experiment was conducted with four different dosages of CaCO addition, 0, 2.25, 4.5 and 7.5 tons/ha, respectively. After one seasonal growth of rice, soil physicochemical properties, soil respiration and bacterial communities were investigated. Results showed that soil pH increased accordingly with increasing dose of CaCO addition, and 7.5 tons/ha addition increased soil pH to neutral condition. Moderate dose of CaCO application (4.5 tons/ha) significantly increased soil dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) content, enhanced soil respiration, while the excessive CaCO application (7.5 tons/ha) decreased these soil properties. High-throughput sequencing results illustrated that moderate dose of CaCO application increased the richness and alpha diversity of soil bacterial community. Compared with control, the relative abundance of Anaerolineaceae family belonging to Chloroflexi phylum increased by 38.7%, 35.4% and 24.5% under 2.25, 4.5 and 7.5 tons/ha treatments, respectively. Redundancy analysis (RDA) showed that soil pH was the most important factor shaping soil bacterial community. The results of this study suggest that proper dose of CaCO additions to acid paddy soil in southern China could have positive effects on soil properties and bacterial community.
碳酸钙（CaCO）的应用广泛用于改善土壤酸化。为了抵消土壤和细菌群落对中国南方酸性稻田中碳酸钙应用的反应，进行了一项田间试验，分别施加了 0、2.25、4.5 和 7.5 吨/公顷的四种不同剂量的碳酸钙。在水稻一个季节的生长后，调查了土壤理化性质、土壤呼吸和细菌群落。结果表明，随着碳酸钙施用量的增加，土壤 pH 值相应增加，7.5 吨/公顷的施用量将土壤 pH 值提高到中性条件。适量的碳酸钙施用（4.5 吨/公顷）显著增加了土壤溶解有机碳（DOC）和溶解有机氮（DON）含量，增强了土壤呼吸，而过量的碳酸钙施用（7.5 吨/公顷）则降低了这些土壤特性。高通量测序结果表明，适量的碳酸钙施用增加了土壤细菌群落的丰富度和α多样性。与对照相比，Anaerolineaceae 科的相对丰度分别增加了 38.7%、35.4%和 24.5%，在 2.25、4.5 和 7.5 吨/公顷处理下，分别属于 Chloroflexi 门。冗余分析（RDA）表明，土壤 pH 值是塑造土壤细菌群落的最重要因素。本研究结果表明，在中国南方酸性稻田中适当施用碳酸钙对土壤性质和细菌群落可能具有积极影响。
