Chung Wen-Hsin, Ishii Hideo, Nishimura Kumiko, Fukaya Masako, Yano Kazutaka, Kajitani Yuji
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan (National Chung Hsing University, Taichung 402, Taiwan, ROC).
National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan.
Plant Dis. 2006 Apr;90(4):506-512. doi: 10.1094/PD-90-0506.
Anthracnose diseases of fruit crops are mainly caused by Colletotrichum gloeosporioides and C. acutatum. In these Colletotrichum species, intra- and interspecific variation in fungicide sensitivity has been reported; however, the relationship between fungicide sensitivity and molecular phylogeny has not been analyzed. Fifty-one isolates from 10 fruit crops, acacia, and tea were tested for their sensitivities to thiophanate-methyl, diethofencarb, and iminoctadine-triacetate, and their internal transcribed spacer (ITS) and 5.8S regions of rDNA were analyzed. C. gloeosporioides isolates were divided into sensitive, less sensitive, intermediate resistant, or resistant to the three fungicides. In contrast, C. acutatum isolates were all less sensitive. In molecular phylogenetic analyses, C. gloeosporioides isolates fell into the same genetic group, whereas C. acutatum isolates were placed into two genetic groups. Although phylogenetic relationship was not closely related to fungicide sensitivity, the isolates of C. gloeosporioides most resistant to iminoctadine-triacetate were found in the same phylogenetic subgroup.
水果作物的炭疽病主要由胶孢炭疽菌和尖孢炭疽菌引起。在这些炭疽菌物种中，已报道了种内和种间对杀菌剂敏感性的差异；然而，杀菌剂敏感性与分子系统发育之间的关系尚未得到分析。对来自10种水果作物、金合欢和茶的51个分离株进行了对甲基托布津、乙霉威和醋酸亚胺唑的敏感性测试，并分析了它们核糖体DNA的内部转录间隔区（ITS）和5.8S区域。胶孢炭疽菌分离株对这三种杀菌剂分为敏感、较不敏感、中度抗性或抗性。相比之下，尖孢炭疽菌分离株均较不敏感。在分子系统发育分析中，胶孢炭疽菌分离株属于同一遗传组，而尖孢炭疽菌分离株被分为两个遗传组。虽然系统发育关系与杀菌剂敏感性没有密切关系，但对醋酸亚胺唑最具抗性的胶孢炭疽菌分离株存在于同一系统发育亚组中。
