College of Biology, Hunan University, Changsha, Hunan 410082, China,
Int J Nanomedicine. 2019 Feb 4;14:937-950. doi: 10.2147/IJN.S193624. eCollection 2019.
Skeletal muscle tissue engineering often involves the prefabrication of muscle tissues in vitro by differentiation and maturation of muscle precursor cells on a platform which provides an environment that facilitates the myogenic differentiation of the seeded cells.
Poly lactic-co-glycolic acid (PLGA) 3D printed scaffolds, which simulate the highly complex structure of extracellular matrix (ECM), were fabricated by E-jet 3D printing in this study. The scaffolds were used as platforms, providing environment that aids in growth, differentiation and other properties of C2C12 myoblast cells.
The C2C12 myoblast cells grown on the PLGA 3D printed platforms had enhanced cell adhesion and proliferation. Moreover, the platforms were able to induce myogenic differentiation of the myoblast cells by promoting the formation of myotubes and up-regulating the expressions of myogenic genes (MyHC and MyOG).
The fabricated 3D printed platforms have excellent biocompatibility, thereby can potentially be used as functional cell culture platforms in skeletal tissue engineering and regeneration.
骨骼肌组织工程通常涉及在体外通过在提供有利于接种细胞成肌分化的环境的平台上对肌肉前体细胞进行分化和成熟来预制肌肉组织。
本研究通过 E-喷射 3D 打印制造了聚乳酸-共-羟基乙酸（PLGA）3D 打印支架，该支架模拟了细胞外基质（ECM）的高度复杂结构。这些支架用作平台，为 C2C12 成肌细胞的生长、分化和其他特性提供了有利的环境。
在 PLGA 3D 打印平台上生长的 C2C12 成肌细胞增强了细胞黏附和增殖。此外，这些平台通过促进肌管的形成和上调成肌基因（MyHC 和 MyOG）的表达，能够诱导成肌细胞的成肌分化。
所制造的 3D 打印平台具有优异的生物相容性，因此可能被用作骨骼肌组织工程和再生中的功能性细胞培养平台。
