Yuan Fei, Liu Jie, Pang Hengyuan, Tian Yu, Yuan Kaikun, Li Yang, Wang Jianjiao, Bian Shan, Zheng Yongri, Dong Deli, Li Yu, Li Mao, Jiang Chuanlu, Hu Shaoshan, Li Qingsong
Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University Harbin 150086, China.
Institute of Molecular Biotechnology of The Austrian Academy of Sciences Austrian.
Am J Transl Res. 2019 Jan 15;11(1):361-369. eCollection 2019.
MicroRNAs (miRNAs) act an important role in the progression of tumor. In this study, we showed that the serum expression of miR-365 was downregulated in the glioblastoma compared with in the healthy controls. We also demonstrated that miR-365 expression was downregulated in glioblastoma tissues compared with the adjacent normal tissues. Overexpression of miR-365 suppressed the glioblastoma cell proliferation and migration. Moreover, ectopic expression of miR-365 promoted the expression of Ecadherin while inhibited the expression of N-cadherin and Vimentin in U87 cell. Furthermore, we identified PAX6 as a direct target gene of miR-365 in U87 cell. Overexpression of miR-365 suppressed glioblastoma cell proliferation and migration and epithelial-to-mesenchymal transition through inhibiting PAX6 expression. These results suggested that miR-365 played a tumor suppressor in glioblastoma.
微小RNA（miRNA）在肿瘤进展中发挥重要作用。在本研究中，我们发现与健康对照相比，胶质母细胞瘤中miR-365的血清表达下调。我们还证明，与相邻正常组织相比，胶质母细胞瘤组织中miR-365表达下调。miR-365的过表达抑制了胶质母细胞瘤细胞的增殖和迁移。此外，miR-365的异位表达促进了E-钙黏蛋白的表达，同时抑制了U87细胞中N-钙黏蛋白和波形蛋白的表达。此外，我们确定PAX6是U87细胞中miR-365的直接靶基因。miR-365的过表达通过抑制PAX6表达抑制了胶质母细胞瘤细胞的增殖、迁移和上皮-间质转化。这些结果表明，miR-365在胶质母细胞瘤中发挥肿瘤抑制作用。
