磷酸组蛋白 H3（PHH3）在滤泡性淋巴瘤分级中的应用：与 Ki-67 和 H&E 有丝分裂计数的比较研究。
The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading: A Comparative Study With Ki-67 and H&E Mitotic Count.
机构信息
Department of Pathology, Brooke Army Medical Center, San Antonio, TX.
出版信息
Am J Clin Pathol. 2019 May 3;151(6):542-550. doi: 10.1093/ajcp/aqz003.
OBJECTIVES
To determine the utility of phosphohistone H3 (PHH3) mitotic count (MC) in grading follicular lymphoma (FL).
METHODS
FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centroblasts/hpf. Results were assessed for correlations and interobserver variability.
RESULTS
Forty-three cases of FL were studied. PHH3 MC resulted in the strongest correlation to grade (r = 0.701, P < .0001) compared with Ki-67 proliferation index (PI) (r = 0.681, P < .0001) and H&E MC (r = 0.536, P = .0002) and the strongest linear relationship to centroblast count (R2 = 0.453). Agreement among pathologists was strongest for PHH3 (intraclass correlation coefficient [ICC] = 0.86) followed by Ki-67 PI (ICC = 0.85) and H&E MC (ICC = 0.78).
CONCLUSIONS
PHH3 correlates best to histologic grade and has less interobserver variability compared with Ki-67 PI and H&E MC. These results support using PHH3 as an adjunct in FL grading.
目的
确定磷酸组蛋白 H3（PHH3）有丝分裂计数（MC）在滤泡性淋巴瘤（FL）分级中的应用价值。
方法
鉴定了 FL 病例（2005-2017 年）。三位血液病理学家记录了他们使用 PHH3 和 H&E 染色的平均 Ki-67 增殖指数、MC/高倍镜视野（hpf）和中心母细胞数/hpf。评估结果的相关性和观察者间变异性。
结果
研究了 43 例 FL 病例。与 Ki-67 增殖指数（PI）（r=0.681，P<.0001）和 H&E MC（r=0.536，P=.0002）相比，PHH3 MC 与分级的相关性最强（r=0.701，P<.0001），与中心母细胞计数的线性关系最强（R2=0.453）。病理学家之间的一致性以 PHH3 最强（组内相关系数[ICC]=0.86），其次是 Ki-67 PI（ICC=0.85）和 H&E MC（ICC=0.78）。
结论
与 Ki-67 PI 和 H&E MC 相比，PHH3 与组织学分级相关性最好，观察者间变异性最小。这些结果支持将 PHH3 作为 FL 分级的辅助手段。
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