Oral Histopathology Laboratory, Faculty of Dentistry University of the Republic, Uruguay
Med Oral Patol Oral Cir Bucal. 2022 Sep 1;27(5):e444-e451. doi: 10.4317/medoral.25420.
The Phosphohistone H3 (PHH3) antibody is recognized as a biomarker of cell proliferation, specific for cells in mitosis, of prognostic value in different malignant neoplasms, however it has been poorly studied in oral squamous cell carcinoma (OSCC). The main objective of this study was to evaluate the immunoexpression of the PHH3 in the OSCC, through the correlation with the immunoexpression of Ki-67, the mitotic activity index (MAI), histological grading, clinical-morphological parameters and the rate of survival.
The study sample consisted of 62 cases of OSCC diagnosed in the Pathological Anatomy Laboratory of the Faculty of Dentistry, University of the Republic (Uruguay). In each of them, an immunohistochemical technique was performed for Ki-67 and PHH3 (serine 10) antibodies. Image J software was used for the MAI and biomarker quantification, defining the percentage of positivity and mitotic figures per 1000 tumor cells.
a significant association was obtained between the expression of PHH3 (p 0.016) and MAI (p 0.031) with survival time. However, no similar relationship was found with Ki-67 (p 0.295). Although it was confirmed a statistical association between histological grade and Ki-67 immunoexpression (p 0.004), PHH3 did not show a similar relationship (p 0.564).
It was confirmed the role of the PHH3 antibody as a biomarker of mitotic figures in OSCC and as a potential marker of cell proliferation. It is noteworthy that this is one of the first works that evaluates a possible relationship between the expression of this antibody and survival in OSCC.
磷酸化组蛋白 H3（PHH3）抗体被认为是细胞增殖的生物标志物，对有丝分裂细胞特异，在不同的恶性肿瘤中具有预后价值，但在口腔鳞状细胞癌（OSCC）中研究甚少。本研究的主要目的是通过与 Ki-67、有丝分裂活动指数（MAI）、组织学分级、临床形态学参数和生存率的相关性，评估 PHH3 在 OSCC 中的免疫表达。
研究样本由乌拉圭共和国大学牙科学院病理解剖实验室诊断的 62 例 OSCC 组成。在每例中，均进行了 Ki-67 和 PHH3（丝氨酸 10）抗体的免疫组织化学技术。使用 Image J 软件进行 MAI 和生物标志物定量，定义阳性百分比和每 1000 个肿瘤细胞的有丝分裂数。
PHH3（p=0.016）和 MAI（p=0.031）的表达与生存时间呈显著相关。然而，与 Ki-67 （p=0.295）无类似关系。尽管证实了组织学分级与 Ki-67 免疫表达之间存在统计学关联（p=0.004），但 PHH3 没有显示出类似的关系（p=0.564）。
证实了 PHH3 抗体作为 OSCC 有丝分裂数生物标志物和潜在增殖细胞标志物的作用。值得注意的是，这是评估该抗体表达与 OSCC 生存率之间可能存在关系的首批研究之一。
