Tikhonov A A, Savvateeva E N, Chernichenko M A, Maslennikov V V, Sidorov D V, Rubina A Yu, Kushlinskii N E
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
P. A. Hertsen Moscow Research Oncological Institute, Affiliated Branch of National Medical Research Center of Radiology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2019 Feb;166(4):489-493. doi: 10.1007/s10517-019-04379-2. Epub 2019 Feb 20.
We propose an approach that allows simultaneous determination of the levels of M and G isotypes of antibodies to the panel of glycans using microarrays. The level of IgG antibodies to 3'-O-su-Le glycan detects patients with colorectal cancer with a sensitivity of 69.77% and specificity of 62.75%. The percentage of correctly classified colorectal cancer patients with the use of a combination of two markers IgM antibodies to glycans 3'-sialyl-TF and 3'-O-su-Le is as high as 74.23%. The levels of IgM antibodies to 3'-O-su-Le glycan differ significantly in patients with and without regional metastases. The levels of some of antiglycan IgM or IgG antibodies differed significantly in patients with tumors of different location and differentiation.
我们提出了一种方法，该方法能够使用微阵列同时测定针对聚糖组的M和G同种型抗体水平。针对3'-O-su-Le聚糖的IgG抗体水平检测结直肠癌患者的灵敏度为69.77%，特异性为62.75%。使用两种标志物（针对聚糖3'-唾液酸-TF和3'-O-su-Le的IgM抗体）组合正确分类的结直肠癌患者百分比高达74.23%。有区域转移和无区域转移的患者中，针对3'-O-su-Le聚糖的IgM抗体水平存在显著差异。在不同位置和分化程度的肿瘤患者中，一些抗聚糖IgM或IgG抗体水平存在显著差异。
