[变应性炎症中抗聚糖IgG和IgM谱的改变]

[Modification of Anti-Glycan IgG and IgM Profiles in Allergic Inflammation].

作者信息

Butvilovskaya V I, Smoldovskaya O V, Feyzkhanova G U, Filippova M A, Pavlushkina L V, Voloshin S A, Rubina A Yu

机构信息

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.

Filatov Moscow Pediatric Clinic no. 13, Healthcare Department of Moscow, Moscow, 123001 Russia.

出版信息

Mol Biol (Mosk). 2018 Jul-Aug;52(4):634-643. doi: 10.1134/S0026898418040031.

DOI:10.1134/S0026898418040031

PMID:30113029

Abstract

Glycans and anti-glycan antibodies (AGAs) are essential for infiltration of inflammatory cells in various allergies. The glycocalyx structure of the cells is modified during disease progression, and this modification is possible to evaluate by assessment of AGAs. A printed glycan array with 55 immobilized glycans and immobilized antibodies to IgG, IgA, and IgM was used to study the changes in AGA profiles in bronchial asthma (BA). Levels of antibodies to certain glycans in BA patients statistically differed from levels in healthy donors (p < 0.0007 by the Mann-Whitney test); the glycan set included 6Su-6`-SiaLec, Sia LeX, Sia6Htype2; Tαα, Manβ1-4GlcNAc, and Manα1-4Manβ. The obtained results help to better understand the mechanisms of the cell-mediated immune response in bronchial asthma and other types of allergic reactions.

摘要

聚糖和抗聚糖抗体（AGAs）对于各种过敏反应中炎症细胞的浸润至关重要。在疾病进展过程中，细胞的糖萼结构会发生改变，并且这种改变可以通过评估AGAs来进行评估。使用一种包含55种固定化聚糖以及针对IgG、IgA和IgM的固定化抗体的印刷聚糖阵列，来研究支气管哮喘（BA）中AGAs谱的变化。BA患者中针对某些聚糖的抗体水平与健康供体的水平在统计学上存在差异（曼-惠特尼检验，p < 0.0007）；聚糖组包括6Su-6`-SiaLec、Sia LeX、Sia6Htype2；Tαα、Manβ1-4GlcNAc和Manα1-4Manβ。所获得的结果有助于更好地理解支气管哮喘和其他类型过敏反应中细胞介导的免疫反应机制。