Unidad de Genómica Avanzada (Langebio), Centro de Investigación y de Estudios Avanzados del IPN, 36824, Irapuato, Guanajuato, Mexico.
Mech Ageing Dev. 2019 Apr;179:36-43. doi: 10.1016/j.mad.2019.02.003. Epub 2019 Feb 18.
Dietary restriction-limitation of calories or other specific nutrients in the diet-is the sole non-genetic intervention known to extend the lifespan of a wide range of model organisms from yeast to mammals. Cell biology studies on the responses to dietary restriction have provided important clues about the mechanisms of longevity; however, a comprehensive genome-wide description of lifespan by dietary restriction has been mostly absent. Large-scale genetic analysis in the budding yeast Saccharomyces cerevisiae offers a great opportunity to uncover the conserved systems-level mechanisms that give way to longevity in response to diet. Here, we review recent advances in high-throughput phenotyping of the replicative and chronological life spans of yeast cells, which have contributed to our understanding of longevity by dietary restriction and the cellular crosstalks of nutrient-sensing regulation.
饮食限制——限制饮食中的卡路里或其他特定营养素——是唯一已知的非遗传干预措施，可以延长从酵母到哺乳动物等多种模式生物的寿命。关于饮食限制响应的细胞生物学研究为长寿机制提供了重要线索；然而，饮食限制对寿命的全基因组描述在很大程度上仍然缺失。出芽酵母酿酒酵母中的大规模遗传分析为揭示响应饮食而导致长寿的保守系统水平机制提供了绝佳机会。在这里，我们回顾了最近在酵母细胞的复制和时序寿命的高通量表型分析方面的进展，这些进展有助于我们理解饮食限制和营养感应调节的细胞串扰对长寿的影响。
