Jones Roger A C, Coutts Brenda A, Mackie Alison E, Dwyer Geoffrey I
Plant Pathology Section, Department of Agriculture, Locked Bag No. 4, Bentley Delivery Centre, WA 6983, Australia.
Saturn Biotech Ltd., State Agricultural Biotechnology Centre, Murdoch University, WA 6150, Australia.
Plant Dis. 2005 Oct;89(10):1048-1050. doi: 10.1094/PD-89-1048.
Under conditions that excluded any possibility of eriophyid mite vector activity, seed transmission of Wheat streak mosaic virus (WSMV) was shown in eight different wheat genotypes at rates of 0.5 to 1.5%. Virus identification in seedlings came from characteristic symptoms in wheat, enzyme-linked immunosorbent assay with WSMV-specific antibodies, reverse-transcription polymerase chain reaction tests with WSMV-specific primers, and cDNA sequence comparisons with published sequences. Sequence comparisons of four seedborne isolates showed ≥98.6% identity with the eight Australian isolates in GenBank, indicating a common seedborne origin of WSMV. These findings warrant reconsideration of currently accepted views on WSMV epidemiology and the likelihood of introducing it to new locations through planting untested wheat seed and the movement of germplasm.
在排除了叶螨传毒可能性的条件下，小麦线条花叶病毒（WSMV）在8种不同的小麦基因型中通过种子传播，传播率为0.5%至1.5%。幼苗中的病毒鉴定来自小麦的特征症状、用WSMV特异性抗体进行的酶联免疫吸附测定、用WSMV特异性引物进行的逆转录聚合酶链反应试验以及与已发表序列的cDNA序列比较。对4个种传分离株的序列比较表明，与GenBank中的8个澳大利亚分离株的同一性≥98.6%，表明WSMV有共同的种传起源。这些发现有必要重新考虑目前关于WSMV流行病学的公认观点，以及通过种植未经检测的小麦种子和种质移动将其引入新地点的可能性。
