Ma Zhonghua, Michailides Themis J
Department of Plant Pathology, University of California-Davis, Kearney Agricultural Center, Parlier 93648.
Plant Dis. 2005 Oct;89(10):1083-1089. doi: 10.1094/PD-89-1083.
The population structure of Botrytis cinerea was investigated by using transposable elements, DNA fingerprinting generated by microsatellite primed-polymerase chain reaction (MP-PCR), and sensitivity to the hydroxyanilide fungicide, fenhexamid, for 234 isolates collected from fig, grape, kiwifruit, pea, and squash in California. Among 234 isolates tested, 195 had two transposable elements, Boty and Flipper (transposa type), 38 had only the Boty element (Boty type), and one had neither of these elements (vacuma type). Four of these 234 isolates, which belonged to the Boty type, were resistant to fenhexamid. A phenogram generated based on MP-PCR markers showed that the isolates were not clustered based on their source hosts or the presence of transposable elements. Analysis of molecular variance (AMOVA) showed that there were no significant genetic differentiations among isolates collected from grape, kiwifruit, pea, and squash at the Kearney Agricultural Center. A more detailed analysis based on AMOVA partition of the total genetic variance indicated that 96% of the variation occurred within populations. The parsimony tree length permutation (PTLPT) and index of association ( I) analyses of MP-PCR data set were consistent with absence of sexual recombination in sampled populations of this pathogen.
利用转座元件、微卫星引物聚合酶链反应（MP-PCR）产生的DNA指纹图谱以及对羟基苯胺类杀菌剂苯酰菌胺的敏感性，对从加利福尼亚州的无花果、葡萄、猕猴桃、豌豆和南瓜中收集的234个灰葡萄孢菌分离株的群体结构进行了研究。在测试的234个分离株中，195个有两个转座元件，即Boty和Flipper（转座类型），38个只有Boty元件（Boty类型），1个既没有这些元件（空泡类型）。这234个分离株中有4个属于Boty类型，对苯酰菌胺具有抗性。基于MP-PCR标记生成的系统发育树状图表明，分离株并非根据其来源宿主或转座元件的存在进行聚类。分子方差分析（AMOVA）表明，在科尔尼农业中心从葡萄、猕猴桃、豌豆和南瓜中收集的分离株之间没有显著的遗传分化。基于总遗传方差的AMOVA划分进行的更详细分析表明，96%的变异发生在种群内部。MP-PCR数据集的简约树长置换（PTLPT）和关联指数（I）分析与该病原菌抽样群体中不存在有性重组一致。
