Phytopathology. 1999 Oct;89(10):967-73. doi: 10.1094/PHYTO.1999.89.10.967.
ABSTRACT Strains of Botrytis cinerea (the anamorph of Botryotinia fuckeliana) were collected from 21 different plant species around vineyards in the Champagne region (France). Strains were analyzed using three new polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) markers that were found by SWAPP (sequencing with arbitrary primer pairs), in addition to 15 other markers (PCR-RFLP, transposable elements, and resistance to fungicides). The markers revealed a high degree of genetic diversity and were used to investigate population structure. The two sympatric species transposa and vacuma, previously identified on grapes in these vineyards, were also detected on many of the plant species sampled. A new type of strain was also detected, having only the transposable element Boty. We did not detect any differentiation between strains from different organs or locations, but the prevalences of transposa and vacuma were significantly different on the different host plants. Fungicide resistance frequencies were significantly different in transposa and vacuma species. This study confirms that B. cinerea is a complex of sibling species and shows that the sibling species occur sympatrically on many host plants. However, the two species seemed to have different pathogenic behaviors. These findings contradict the traditional view of B. cinerea as a clonal population without specialization.
摘要 从香槟地区（法国）葡萄园周围的 21 种不同植物上采集了 Botrytis cinerea（Botryotinia fuckeliana 的无性型）菌株。使用 SWAPP（任意引物对测序）发现的 3 种新的聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）标记以及其他 15 种标记（PCR-RFLP、转座子和抗真菌剂）对菌株进行了分析。这些标记揭示了高度的遗传多样性，并用于研究种群结构。先前在这些葡萄园的葡萄上发现的两种同域种 transposa 和 vacuma 也在许多采样的植物物种上检测到。还检测到了一种新型菌株，仅具有转座子 Boty。我们没有检测到来自不同器官或位置的菌株之间的任何分化，但 transposa 和 vacuma 在不同的宿主植物上的流行率有显著差异。转座子和 vacuma 物种中的抗真菌剂抗性频率有显著差异。本研究证实了 Botrytis cinerea 是一个姊妹种复合体，并表明在许多宿主植物上存在同域的姊妹种。然而，这两个物种似乎具有不同的致病行为。这些发现与 Botrytis cinerea 作为无特化的无性种群的传统观点相矛盾。
