Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
Demethra Biotech, Strada dell'Innovazione 1, Camisano Vicentino, 36043 Vicenza, Italy.
Int J Mol Sci. 2019 Feb 19;20(4):897. doi: 10.3390/ijms20040897.
Kiwifruit ( cv. Hayward) is a commercially important crop with highly nutritional green fleshy fruits. The post-harvest maturation of the fruits is well characterized, but little is known about the metabolic changes that occur during fruit development. Here we used untargeted metabolomics to characterize the non-volatile metabolite profile of kiwifruits collected at different time points after anthesis, revealing profound metabolic changes before the onset of ripening including the depletion of many classes of phenolic compounds. In contrast, the phytohormone abscisic acid accumulated during development and ripening, along with two indolamines (serotonin and its precursor tryptamine), and these were monitored in greater detail by targeted metabolomics. The role of indolamines in kiwifruit development is completely unknown, so we also characterized the identity of genes encoding tryptophan decarboxylase in and its close relative to provide insight into the corresponding biological processes. Our results indicate that abscisic acid and indolamines fulfill unrecognized functions in the development and ripening of kiwifruits.
奇异果（ cv. Hayward）是一种具有高营养价值的绿色肉质水果，是一种商业上重要的作物。果实的采后成熟过程已经得到很好的描述，但对果实发育过程中发生的代谢变化知之甚少。在这里，我们使用非靶向代谢组学来描述授粉后不同时间点采集的奇异果的非挥发性代谢物图谱，揭示了在成熟开始之前发生的深刻代谢变化，包括许多类酚化合物的消耗。相比之下，脱落酸在发育和成熟过程中积累，以及两种吲哚胺（血清素及其前体色氨酸），通过靶向代谢组学更详细地监测到这些物质。吲哚胺在奇异果发育中的作用完全未知，因此我们还对 和其近亲 中编码色氨酸脱羧酶的基因的身份进行了描述，以深入了解相应的生物学过程。我们的结果表明，脱落酸和吲哚胺在奇异果的发育和成熟过程中发挥了未被认识的作用。
