Kumar Rahul
Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad Hyderabad, India.
Front Plant Sci. 2016 Aug 23;7:1268. doi: 10.3389/fpls.2016.01268. eCollection 2016.
Type II pyridoxal phosphate-dependent decarboxylase (PLP_deC) enzymes play important metabolic roles during nitrogen metabolism. Recent evolutionary profiling of these genes revealed a sharp expansion of histidine decarboxylase genes in the members of Solanaceae family. In spite of the high sequence homology shared by PLP_deC orthologs, these enzymes display remarkable differences in their substrate specificities. Currently, limited information is available on the gene repertoires and substrate specificities of PLP_deCs which renders their precise annotation challenging and offers technical challenges in the immediate identification and biochemical characterization of their full gene complements in plants. Herein, we explored their evolutionary trails in a comprehensive manner by taking advantage of high-throughput data accessibility and computational approaches. We discussed the premise that has enabled an improved reconstruction of their evolutionary lineage and evaluated the factors offering constraints in their rapid functional characterization, till date. We envisage that the synthesized information herein would act as a catalyst for the rapid exploration of their biochemical specificity and physiological roles in more plant species.
II型磷酸吡哆醛依赖性脱羧酶（PLP_deC）在氮代谢过程中发挥着重要的代谢作用。最近对这些基因的进化分析表明，茄科植物成员中的组氨酸脱羧酶基因急剧扩增。尽管PLP_deC直系同源物具有高度的序列同源性，但这些酶在底物特异性方面表现出显著差异。目前，关于PLP_deC的基因库和底物特异性的信息有限，这使得它们的精确注释具有挑战性，并在植物中直接鉴定其完整基因互补体及其生化特性方面带来了技术挑战。在此，我们利用高通量数据的可及性和计算方法，全面探索了它们的进化轨迹。我们讨论了能够改进其进化谱系重建的前提，并评估了迄今为止在其快速功能表征中存在限制的因素。我们设想，本文综合的信息将成为在更多植物物种中快速探索其生化特异性和生理作用的催化剂。
