1 Department of Pediatric Cardiothoracic Surgery, Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
2 Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 200011, China.
J Biomater Appl. 2019 May;33(10):1301-1313. doi: 10.1177/0885328219831055. Epub 2019 Feb 21.
The biocompatibility and bioactivity of injectable acellular extracellular matrix nominates its use as an optimal candidate for cell delivery, serving as a reconstructive scaffold. In this study, we investigated the feasibility of preparing a blood vessel matrix (BVM) hydrogel, which revealed its pro-angiogenic effects in vitro and its therapeutic effects in an in vivo skin flap model. Aortic and abdominal aortic arteries from pigs were acellularized by Triton-X 100 and confirmed by hematoxylin and eosin and 4,6-diamidino-2-phenylindole staining. Different concentrations of blood vessel matrix hydrogel were generated successfully through enzymatic digestion, neutralization, and gelation. Hematoxylin and eosin staining, Masson's trichrome staining, collagen type I immunohistochemistry staining, and enzyme-linked immunosorbent assays showed that type I collagen and some growth factors were retained in the hydrogel. Scanning electron microscopy demonstrated the different diametric fibrils in blood vessel matrix hydrogels. A blood vessel matrix hydrogel-coated plate promoted the tube formation of human umbilical vein endothelial cells in vitro. After injection into skin flaps, the hydrogel improved the flap survival rate and increased blood perfusion and capillary density. These results indicated that we successfully prepared a blood vessel matrix hydrogel and demonstrated its general characteristics and angiogenic effects in vitro and in vivo.
注射用去细胞细胞外基质的生物相容性和生物活性使其成为细胞递送的理想候选物，可用作重建支架。本研究通过 Triton-X 100 对猪的胸主动脉和腹主动脉进行去细胞化处理，并用苏木精和伊红以及 4,6-二脒基-2-苯基吲哚染色进行确认。通过酶消化、中和和凝胶化成功制备了不同浓度的血管基质水凝胶。苏木精和伊红染色、Masson 三色染色、I 型胶原免疫组织化学染色和酶联免疫吸附试验显示，水凝胶中保留了 I 型胶原和一些生长因子。扫描电子显微镜显示了血管基质水凝胶中的不同直径纤维。血管基质水凝胶包被板促进了人脐静脉内皮细胞的管腔形成。注入皮瓣后，水凝胶提高了皮瓣存活率并增加了血液灌注和毛细血管密度。这些结果表明，我们成功制备了血管基质水凝胶，并证明了其在体外和体内的一般特性和血管生成作用。
