de Christo Scherer Marcella Malavazi, Marques Franciane Martins, Figueira Mariana Moreira, Peisino Maria Carolina Oliveira, Schmitt Elisângela Flávia Pimentel, Kondratyuk Tamara P, Endringer Denise Coutinho, Scherer Rodrigo, Fronza Marcio
Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Produtos Naturais, Universidade Vila Velha, CEP 29102-920, Brazil.
Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI, 96720, USA.
J Tissue Viability. 2019 May;28(2):94-99. doi: 10.1016/j.jtv.2019.02.003. Epub 2019 Feb 14.
This study was undertaken to investigate the in vitro wound healing effects and the anti-inflammatory and antioxidant activities of terpinolene and α-phellandrene. The in vitro stimulatory effects on the proliferation and migration of fibroblasts were assessed using the scratch assay. The anti-inflammatory activity was evaluated using cell-based assays by investigating their influence on nitric oxide (NO), superoxide anion (O), tumour necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) production and using the TNF-α-induced nuclear factor kappa (NF-κB) assay. Antioxidant activity was determined by the ABTS cation radical scavenging capacity, ferric reducing/antioxidant potential (FRAP), and NO free radical scavenging assays. Terpinolene and α-phellandrene significantly increased the migration and proliferation of fibroblasts and suppressed the pro-inflammatory cytokines IL-6 and TNF-α in a dose-dependent manner. Terpinolene and α-phellandrene at a concentration of 100 μM significantly inhibited NO production (41.3 and 63.8%, respectively) in a macrophage cell-culture-based assay, and resulted in reductions in O production of 82.1 ± 3.5% and 70.6 ± 4.3%, respectively. Moreover, these monoterpenes were verified to suppress NF-κB activity. In summary, terpinolene and α-phellandrene may contribute to broadening clinical options in the treatment of wounds by attenuating inflammation and oxidative stress in vitro.
本研究旨在探讨萜品油烯和α-水芹烯的体外伤口愈合作用以及抗炎和抗氧化活性。使用划痕试验评估其对成纤维细胞增殖和迁移的体外刺激作用。通过研究它们对一氧化氮（NO）、超氧阴离子（O）、肿瘤坏死因子-α（TNF-α）和白细胞介素6（IL-6）产生的影响，并使用TNF-α诱导的核因子κB（NF-κB）试验，采用基于细胞的试验评估抗炎活性。通过ABTS阳离子自由基清除能力、铁还原/抗氧化能力（FRAP）和NO自由基清除试验测定抗氧化活性。萜品油烯和α-水芹烯显著增加成纤维细胞的迁移和增殖，并以剂量依赖的方式抑制促炎细胞因子IL-6和TNF-α。在基于巨噬细胞培养的试验中，浓度为100μM的萜品油烯和α-水芹烯分别显著抑制NO产生（分别为41.3%和63.8%），并分别导致O产生减少82.1±3.5%和70.6±4.3%。此外，这些单萜被证实可抑制NF-κB活性。总之，萜品油烯和α-水芹烯可能通过在体外减轻炎症和氧化应激，有助于拓宽伤口治疗的临床选择。
