Khassanova Gulmira, Kurishbayev Akhylbek, Jatayev Satyvaldy, Zhubatkanov Askar, Zhumalin Aybek, Turbekova Arysgul, Amantaev Bekzak, Lopato Sergiy, Schramm Carly, Jenkins Colin, Soole Kathleen, Langridge Peter, Shavrukov Yuri
Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Astana, Kazakhstan.
Biological Sciences, College of Science and Engineering, Flinders University, Bedford Park, SA, Australia.
Front Genet. 2019 Feb 7;10:40. doi: 10.3389/fgene.2019.00040. eCollection 2019.
Intracellular vesicle trafficking genes, , encoding small GTP binding proteins, have been well studied in medical research, but there is little information concerning these proteins in plants. Some sub-families of the genes have not yet been characterized in plants, such as - otherwise known as in yeast and animals. Our study aimed to identify all gene sequences in chickpea ( L.) using bioinformatics approaches, with a particular focus on the gene sub-family since it featured in an SNP database. Five isoforms of the gene were identified and studied: and . Six accessions of both Desi and Kabuli ecotypes, selected from field trials, were tested for tolerance to abiotic stresses, including salinity, drought and rapid dehydration and compared to plant growth under control conditions. Expression analysis of total and individual isoforms in leaves of control plants revealed a very high level of expression, with the greatest contribution made by . Salinity stress (150 mM NaCl, 12 days in soil) caused a 2-3-fold increased expression of total compared to controls, with the highest expression in isoforms and . Significantly decreased expression of all five isoforms of was observed under drought (12 days withheld water) compared to controls. In contrast, both total and the isoform showed very high expression (up-to eight-fold) in detached leaves over 6 h of dehydration. The results suggest that the gene is involved in plant growth and response to abiotic stresses. It was highly expressed in leaves of non-stressed plants and was down-regulated after drought, but salinity and rapid dehydration caused up-regulation to high and very high levels, respectively. The isoforms of were differentially expressed, with the highest levels recorded for in controls and under salinity stress, and for - in rapidly dehydrated leaves. Genotypic variation in , comprising eleven SNPs, was found through sequencing of the local chickpea cultivar Yubileiny and germplasm ICC7255 in comparison to the two fully sequenced reference accessions, ICC4958 and Frontier. Amplifluor-like markers based on one of the identified SNPs in were designed and successfully used for genotyping chickpea germplasm.
编码小GTP结合蛋白的细胞内囊泡运输基因在医学研究中已得到充分研究，但在植物中关于这些蛋白的信息却很少。该基因的一些亚家族在植物中尚未得到表征，例如——在酵母和动物中也称为。我们的研究旨在利用生物信息学方法鉴定鹰嘴豆（L.）中的所有基因序列，特别关注基因亚家族，因为它出现在一个SNP数据库中。鉴定并研究了该基因的五种异构体：和。从田间试验中选择的六个迪西和卡布利生态型品种，测试了它们对非生物胁迫的耐受性，包括盐度、干旱和快速脱水，并与对照条件下的植物生长进行了比较。对照植物叶片中总异构体和单个异构体的表达分析显示表达水平非常高，其中贡献最大。盐胁迫（150 mM NaCl，土壤中12天）导致总表达量比对照增加2至3倍，异构体和中的表达最高。与对照相比，在干旱（停水12天）条件下观察到所有五种异构体的表达均显著降低。相反，在脱水6小时的离体叶片中，总和异构体均显示出非常高的表达（高达八倍）。结果表明，该基因参与植物生长和对非生物胁迫的响应。它在未受胁迫的植物叶片中高度表达，干旱后下调，但盐度和快速脱水分别导致上调至高水平和非常高水平。异构体差异表达，在对照和盐胁迫下记录到的最高水平为，在快速脱水叶片中为。通过对当地鹰嘴豆品种尤比列尼和种质ICC7255进行测序，与两个完全测序的参考品种ICC4958和边疆相比，发现了包含11个SNP的基因分型变异。基于鉴定出的基因中的一个SNP设计了类似Amplifluor的标记，并成功用于鹰嘴豆种质的基因分型。
