Associate Professor, Department of Oral Anatomy, Dental Research Institute and Seoul National University School of Dentistry, Seoul, Republic of Korea.
Biomed Res Int. 2019 Jan 17;2019:7905404. doi: 10.1155/2019/7905404. eCollection 2019.
Histomorphometry is the established gold standard for inspection of trabecular microstructures in biomaterial research. However, microcomputed tomography can provide images from the perspective of various section planes. The aim of the present study was to evaluate the effects of different section planes, which may cause bias in two-dimensional morphometry, on the morphometric values of microcomputed tomography.
A socket preservation technique was performed on the extracted premolar area of 4 beagle dogs. After an 8-week healing period, a total of 16 specimens were obtained and analyzed with conventional histomorphometry and microtomographic morphometry. Using the original images of the histologic specimens for comparison, the most similar tomographic image was selected by trial and error. Then, the section plane was then moved with ±79 m parallel offsets and rotated ±10° around the center from the occlusal view. The images were compared in terms of bone, graft, and noncalcified area, and the concordance correlation coefficient (CCC) was calculated.
There was a high CCC in the comparison between histomorphometric images and the most similar microtomographic images. However, the CCC value was low in the comparisons with both parallel movement and rotation. Our results demonstrate that the sectioning plane has a significant effect on measurements.
Two-dimensional morphometric values for biomaterial research should be interpreted with caution, and the simultaneous use of complementary 3-dimensional tools is recommended.
组织形态计量学是生物材料研究中检查小梁微结构的既定金标准。然而，微计算机断层扫描可以从各种层面提供图像。本研究的目的是评估不同层面的影响，这些层面可能会导致二维形态计量学中的偏差，从而对微计算机断层扫描的形态计量值产生影响。
在 4 只比格犬的前磨牙区进行了牙槽窝保存技术。经过 8 周的愈合期，共获得 16 个标本，分别进行常规组织形态计量学和微断层形态计量学分析。通过反复试验，使用组织学标本的原始图像进行比较，选择最相似的断层图像。然后，将层面沿咬合方向以±79μm 的平行偏移和平移±10°的角度进行移动。比较骨、移植物和未钙化区域的图像，并计算一致性相关系数（CCC）。
组织形态计量学图像与最相似的微断层图像之间的一致性相关系数（CCC）较高。然而，与平行移动和旋转相比，CCC 值较低。我们的结果表明，层面的选择对测量结果有显著影响。
在进行生物材料研究的二维形态计量值时应谨慎解释，建议同时使用互补的三维工具。
