Hemmatzadeh F, Niap F, Bennett B A, Trott D J, Peaston A E
School of Animal and Veterinary Science, The University of Adelaide, Adelaide, SA, Australia.
Lett Appl Microbiol. 2019 May;68(5):409-414. doi: 10.1111/lam.13139. Epub 2019 Mar 19.
The aim of the study was to develop a quantitative real-time PCR assay for diagnosis and monitoring of mycoplasma urinary tract infections (UTI) in a dog. An English Cocker Spaniel dog with the history of urinary tract infection was physically examined and laboratory findings identified chronic renal insufficiency and urinary tract infection. Attempts to culture organisms from pyuric urine failed, and empirical antibiotic therapy did not resolve the pyuria. A mycoplasma species most closely resembling Ureaplasma canigenitalium was identified in urine samples by conventional PCR and sequencing. A quantitative PCR method was developed to monitor and finally verify successful treatment. This novel approach to monitoring mycoplasma urinary tract infections is conceptually simple, and provides rapid results. It may have wider application in monitoring treatment efficacy for infections with other Mycoplasma spp. as well as additional organisms that are difficult to culture. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, we highlight two different findings, detection of Ureaplasma canigenitalium in a dog with chronic urinary tract infection and development of a quantitative real-time PCR test to track treatment results in an infected dog. This report is the first report of detection of U. canigenitalium in one dog in Australia. This novel qPCR method for monitoring mycoplasma urinary tract infections is conceptually simple and provides results fast. It will have wider applications in monitoring treatment efficacy for infections with mycoplasmas and mycoplasma-like organisms that are difficult to culture, and provides a sensitive guide to treatment progress.
本研究的目的是开发一种定量实时PCR检测方法，用于诊断和监测犬支原体性尿路感染（UTI）。对一只患有尿路感染病史的英国可卡犬进行了体格检查，实验室检查发现其存在慢性肾功能不全和尿路感染。从脓性尿液中培养微生物的尝试失败，经验性抗生素治疗未能消除脓尿。通过常规PCR和测序在尿液样本中鉴定出一种与犬脲原体最相似的支原体。开发了一种定量PCR方法来监测并最终验证治疗是否成功。这种监测支原体性尿路感染的新方法概念简单，且能快速得出结果。它可能在监测其他支原体属感染以及其他难以培养的微生物感染的治疗效果方面有更广泛的应用。研究的意义和影响：在本研究中，我们突出了两个不同的发现，即在一只患有慢性尿路感染的犬中检测到犬脲原体，以及开发了一种定量实时PCR检测方法来跟踪感染犬的治疗结果。本报告是澳大利亚首次在一只犬中检测到犬脲原体的报告。这种用于监测支原体性尿路感染的新型qPCR方法概念简单且结果得出快速。它将在监测支原体和类支原体等难以培养的微生物感染的治疗效果方面有更广泛的应用，并为治疗进展提供敏感的指导。
