Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture , Shanghai Academy of Agricultural Sciences , 1000 Jingqi Road , Shanghai 201403 , P. R. China.
College of Pharmaceutical Sciences , Zhejiang University , 866 Yuhangtang Road , Hangzhou 310058 , P. R. China.
Anal Chem. 2019 Mar 19;91(6):4116-4123. doi: 10.1021/acs.analchem.8b05791. Epub 2019 Mar 6.
An innovative approach based on a surface functional monomer-directing strategy for the construction of a sensitive and selective molecularly imprinted electrochemical sensor for patulin recognition is described. A patulin imprinted platinum nanoparticle (PtNP)-coated poly(thionine) film was grown on a preformed thionine tailed surface of PtNP-nitrogen-doped graphene (NGE) by electropolymerization, which provided high capacity and fast kinetics to uptake patulin molecules. Thionine acted not only as a functional monomer for molecularly imprinted polymer (MIP), but also as a signal indicator. Enhanced sensitivity was obtained by combining the excellent electric conductivity of PtNPs, NGE, and thionine with multisignal amplification. The designed sensor displayed excellent performance for patulin detection over the range of 0.002-2 ng mL (R = 0.995) with a detection limit of 0.001 ng mL for patulin. In addition, the resulting sensor showed good stability and high repeatability and selectivity. Furthermore, the feasibility of its applications has also been demonstrated in the analysis of real samples, providing novel tactics for the rational design of MIP-based electrochemical sensors to detect a growing number of deleterious substances.
基于表面功能单体导向策略的创新方法被用于构建用于盘菌素识别的敏感和选择性分子印迹电化学传感器。通过电聚合在预先形成的 PtNP-氮掺杂石墨烯 (NGE) 的硫堇尾部表面上生长了盘菌素印迹铂纳米颗粒 (PtNP) 涂层的聚硫堇膜，这为盘菌素分子的摄取提供了高容量和快速动力学。硫堇不仅作为分子印迹聚合物 (MIP) 的功能单体，而且作为信号指示剂。通过结合 PtNPs、NGE 和硫堇的优异导电性以及多信号放大，获得了增强的灵敏度。设计的传感器在 0.002-2 ng mL 的范围内对盘菌素检测表现出优异的性能（R = 0.995），检测限为 0.001 ng mL。此外，所得传感器表现出良好的稳定性、高重复性和选择性。此外，还已经证明了其在实际样品分析中的可行性，为基于 MIP 的电化学传感器的合理设计提供了检测越来越多有害物质的新策略。
