IEEE Trans Biomed Eng. 2019 Nov;66(11):3080-3087. doi: 10.1109/TBME.2019.2900251. Epub 2019 Feb 20.
In this paper, a new method was established to monitor multichannel neural activity with microelectrode arrays (MEAs) under modulation of caged compounds in a rat model of seizures.
The 16-channel MEAs were fabricated and implanted into the hippocampus of normal rats and epileptic rats for neural spike and local field potential (LFP) recording. Using optical fibers with drug delivery tubing, two different caged compounds [ruthenium-bipyridine-trimethylphosphine glutamate (RuBi-Glu) and ruthenium-bipyridine-trimethylphosphine gamma aminobutyric acid (RuBi-GABA)] were applied, and blue light (465 nm) was used to modulate neural activity.
In normal rats, RuBi-Glu excited neural activity, and RuBi-GABA inhibited neural activity. The amplitude of spikes increased 26% from 154 to 194 μV with RuBi-Glu modulation. During RuBi-GABA modulation, spikes recovered to 142 μV. The firing rate increased from 1.4 to 4.5 Hz with RuBi-Glu modulation and decreased to 0.8 Hz after RuBi-GABA modulation. The power of LFPs increased from 566 to 1128 μW with RuBi-Glu modulation and recovered to 710 μW with RuBi-GABA modulation. In epileptic rats, the neural activity during seizures was significantly inhibited by RuBi-GABA modulation. The amplitude of spikes was 242 μV during seizures and decreased to 112 μV with RuBi-GABA modulation. The firing rate decreased from 20.29 to 1.33 Hz with RuBi-GABA modulation.
Using MEAs, the modulation of neural activity with caged compound photolysis was observed with high temporal-spatial resolution in normal and epileptic rats.
This new method is important for monitoring neural activity with photo-switchable modulation.
本文建立了一种新方法，可在光解笼状化合物调制下，通过微电极阵列（MEA）监测大鼠癫痫模型中的多通道神经活动。
制备 16 通道 MEA 并植入正常和癫痫大鼠的海马中，用于神经峰和局部场电位（LFP）记录。使用带有药物输送管的光纤，施加两种不同的笼状化合物[钌联吡啶三甲膦谷氨酸（RuBi-Glu）和钌联吡啶三甲膦γ-氨基丁酸（RuBi-GABA）]，并用蓝光（465nm）调制神经活动。
在正常大鼠中，RuBi-Glu 兴奋神经活动，而 RuBi-GABA 抑制神经活动。用 RuBi-Glu 调制时，峰的幅度从 154μV 增加到 194μV，增加了 26%。在 RuBi-GABA 调制时，峰恢复到 142μV。用 RuBi-Glu 调制时，放电率从 1.4Hz 增加到 4.5Hz，用 RuBi-GABA 调制时降低到 0.8Hz。用 RuBi-Glu 调制时，LFP 的功率从 566μW 增加到 1128μW，用 RuBi-GABA 调制时恢复到 710μW。在癫痫大鼠中，RuBi-GABA 调制显著抑制癫痫发作时的神经活动。用 RuBi-GABA 调制时，峰的幅度从癫痫发作时的 242μV 降低到 112μV。用 RuBi-GABA 调制时，放电率从 20.29Hz 降低到 1.33Hz。
使用 MEA，在正常和癫痫大鼠中以高时空分辨率观察到用笼状化合物光解调制神经活动。
这种新方法对于用光开关调制监测神经活动很重要。
