微 LED 探针介导的笼状谷氨酸光药理学调节海马局部场电位。
Photopharmacological modulation of hippocampal local field potential by caged-glutamate with MicroLED probe.
机构信息
Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan.
Division for Memory and Cognitive Function, Research Center for Advanced Medical Science, Comprehensive Research Facilities for Advanced Medical Science, Dokkyo Medical University, Tochigi, Japan.
出版信息
Neuropsychopharmacol Rep. 2024 Sep;44(3):658-662. doi: 10.1002/npr2.12472. Epub 2024 Aug 9.
AIM
Photopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light irradiation-dependent manner. Here, we investigated whether a microscale light-emitting diode (MicroLED) probe is applicable for the photoconversion of caged-glutamate (caged-Glu) in vivo.
METHODS
A needle-shaped MicroLED probe was fabricated and inserted into the mouse hippocampal dentate gyrus (DG) with a cannula for drug injection and a recording electrode for measuring the local field potential (LFP). Artificial cerebrospinal fluid (ACSF) or caged-Glu was infused into the DG and illuminated with light from a MicroLED probe.
RESULTS
In the caged-Glu-injected DG, the LFP changed in the 10-20 Hz frequency ranges after light illumination, whereas there was no change in the ACSF control condition.
CONCLUSION
The MicroLED probe is applicable for photopharmacological experiments to modulate LFP with caged-Glu in vivo.
目的
光药理学是一种通过在特定目标区域内特定时间将物质光转化来调节生物现象的新技术。笼状化合物被认为与光药理学兼容，因为未笼状配体以光照射依赖的方式释放并发挥功能。在这里，我们研究了微发光二极管（MicroLED）探头是否适用于体内笼状谷氨酸（caged-Glu）的光转化。
方法
制作了一种针状的 MicroLED 探头，并通过套管将其插入小鼠海马齿状回（DG）中，用于药物注射和测量局部场电位（LFP）的记录电极。人工脑脊液（ACSF）或 caged-Glu 被注入 DG 并用光从 MicroLED 探头照射。
结果
在 caged-Glu 注射的 DG 中，光照射后 LFP 在 10-20 Hz 频率范围内发生变化，而在 ACSF 对照条件下没有变化。
结论
MicroLED 探头适用于体内用 caged-Glu 调节 LFP 的光药理学实验。
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