Centre for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg, Breisacher Straße 115 B, 79106 Freiburg, Germany.
Research Centre skinitial, Department of Dermatology, University Medical Center, Hauptstraße 7, 79104 Freiburg, Germany.
J Ethnopharmacol. 2019 May 10;235:415-423. doi: 10.1016/j.jep.2019.02.034. Epub 2019 Feb 19.
The improvement of wound healing has always been an important issue for both ethnopharmacological and modern medical research. In this study, we used state-of-the-art methods to investigate extracts of plants used traditionally in Nepal for more than 1000 years to treat inflammatory injuries.
We focused on the potential of the plant extracts to ameliorate wound healing and to influence immune modulatory properties.
Nine Nepalese plant extracts in three different solvents (methanol, ethyl acetate, petroleum ether) were immunologically characterised. Water-soluble tetrazolium (WST-1) assays and scratch assays were performed to determine their impact on viability and wound healing capacity of human keratinocytes and fibroblasts. Effects on proliferation, viability and function of physiologically relevant anti-CD3 and anti-CD28 stimulated primary human T lymphocytes were assessed using carboxyfluorescein succinimidyl ester (CFSE), annexin V/propidium iodide staining assays and flow cytometry-based surface receptor characterisation. The secretion level of interleukin-2 (IL-2) was analysed with the ELISA technique. Dendritic cells were generated out of peripheral blood mononuclear cells (PBMC) by CD14 magnetic bead selection. Flow cytometry-based surface receptor characterisation and ELISA-based technique were used to evaluate the DC activation state and the interleukin-8 (IL-8) secretion level.
We demonstrate that an ethyl acetate extract of Bassia longifolia and of Gmelina arborea have anti-inflammatory capacities, indicated by reduced proliferation, inhibition of IL-2 secretion and degranulation capacity of activated human T cells, when compared with adequate concentrations of synthetic positive drug controls. Furthermore, Gmelina arborea improved the wound healing of keratinocytes and fibroblasts and has tendency to increase the secretion of IL-8 by human primary dendritic cells.
With this preliminary screening, we offer a scientific basis for the immunomodulatory properties of the two Nepalese medicinal plants Bassia longifolia and Gmelina arborea. However, further detailed studies regarding the responsible compounds are necessary.
改善伤口愈合一直是民族药理学和现代医学研究的重要问题。在这项研究中，我们使用最先进的方法来研究尼泊尔传统上使用了 1000 多年来治疗炎症损伤的植物提取物。
我们专注于植物提取物改善伤口愈合和影响免疫调节特性的潜力。
对三种不同溶剂（甲醇、乙酸乙酯、石油醚）中的 9 种尼泊尔植物提取物进行了免疫特性研究。采用水溶性噻唑蓝（WST-1）测定法和划痕测定法，研究其对人角质形成细胞和成纤维细胞活力和伤口愈合能力的影响。采用羧基荧光素琥珀酰亚胺酯（CFSE）、 Annexin V/碘化丙啶染色测定法和基于流式细胞术的表面受体特征分析，评估对生理相关抗-CD3 和抗-CD28 刺激的原代人 T 淋巴细胞增殖、活力和功能的影响。采用 ELISA 技术分析白细胞介素-2（IL-2）的分泌水平。通过 CD14 磁珠选择从外周血单核细胞（PBMC）中生成树突状细胞。采用基于流式细胞术的表面受体特征分析和基于 ELISA 的技术，评估 DC 激活状态和白细胞介素-8（IL-8）的分泌水平。
我们证明，与适当浓度的合成阳性药物对照相比， Bassia longifolia 和 Gmelina arborea 的乙酸乙酯提取物具有抗炎作用，表现为增殖减少、抑制 IL-2 分泌和激活的人 T 细胞脱颗粒能力。此外，Gmelina arborea 改善了角质形成细胞和成纤维细胞的伤口愈合，并具有增加人原代树突状细胞分泌 IL-8 的趋势。
通过这项初步筛选，我们为尼泊尔药用植物 Bassia longifolia 和 Gmelina arborea 的免疫调节特性提供了科学依据。然而，还需要进一步详细研究负责化合物。
