Cox N A, Mercuri A J
Animal Products Laboratory, Richard B. Russell Agricultural Research Center, United States Department of Agriculture, Agricultural Research Service, Athens, Georgia 30604.
J Food Prot. 1978 Jul;41(7):521-524. doi: 10.4315/0362-028X-41.7.521.
Each of four serotypes of Salmonella ( S. anatum , S. montevideo , S. saint-paul , S. typhimurium ), inoculated at low levels on broiler carcasses (ca. 20 cells/carcass) was detected by direct enrichment of the whole carcass rinse fluid with either Selenite Cystine Broth (SC) or Selenite Brilliant Green Broth (SGB). Neither Selenite Brilliant Green Sulfa Broth (SBGS) nor TT Broth was effective in detecting the serotypes unless the entire broiler carcass with the rinse fluid was incubated with either of these enrichment broths. SBG and SC were effective as direct enrichment broths for recovering pure cultures of the four seroytpes subjected to sublethal heat treatment (53 C for 1 min) approximating that to which broiler carcasses are subjected during the commercial scalding process.
通过用亚硒酸盐胱氨酸肉汤（SC）或亚硒酸盐煌绿肉汤（SGB）直接富集整个屠体冲洗液，可检测出接种在肉鸡屠体上低水平的四种血清型沙门氏菌（鸭沙门氏菌、蒙得维的亚沙门氏菌、圣保罗沙门氏菌、鼠伤寒沙门氏菌）（约20个细胞/屠体）。除非将带有冲洗液的整个肉鸡屠体与这些增菌肉汤之一一起培养，亚硒酸盐煌绿磺胺肉汤（SBGS）和TT肉汤在检测这些血清型时均无效。SBG和SC作为直接增菌肉汤，对于从经过亚致死热处理（53℃ 1分钟）的四种血清型中回收纯培养物有效，这种热处理近似于商业烫毛过程中肉鸡屠体所经历的处理。
