Izat A L, Driggers C D, Colberg M, Reiber M A, Adams M H
Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville, Arkansas 72701.
J Food Prot. 1989 Aug;52(8):564-570. doi: 10.4315/0362-028X-52.8.564.
In a series of five experiments a total of 269 broiler carcass and chill tank water samples were tested for the presence of Salmonella using the DNA probe and the standard cultural method. Carcasses were sampled using a whole carcass rinse technique. Samples consisted of pre- (48) and post-chill (103) carcasses, and pre-chill (48) and chill (70) tank water. Samples to be evaluated with the DNA probe were subjected to three preenrichment/enrichment procedures to determine the most accurate and reliable enrichment procedure to use with the DNA probe assay. Direct enrichment in Selenite Cystine followed by 24 h incubation in Gram Negative broth allowed for recovery of 0.03 salmonellae/ml from carcass rinse and from pre-chill water using either the standard cultural method or the DNA probe. Preenrichment in Lactose broth produced inaccurate results for pre-chill carcass and pre-chill water samples using the DNA probe assay, and may be due to extreme microbiological competition. No false positive results were obtained using the DNA probe assay for any of the four sample types.
在一系列五项实验中，共对269份肉鸡胴体和冷却槽水样进行了检测，采用DNA探针和标准培养方法检测沙门氏菌的存在。胴体采用全胴体冲洗技术进行采样。样本包括预冷（48份）和冷却后（103份）的胴体，以及预冷（48份）和冷却（70份）的槽水。用DNA探针评估的样本要经过三个预富集/富集程序，以确定与DNA探针检测法一起使用的最准确、最可靠的富集程序。在亚硒酸盐胱氨酸中直接富集，然后在革兰氏阴性肉汤中培养24小时，使用标准培养方法或DNA探针，从胴体冲洗液和预冷水样中可回收0.03个/ml的沙门氏菌。对于预冷胴体和预冷水样，使用DNA探针检测法时，在乳糖肉汤中进行预富集得到的结果不准确，这可能是由于极端的微生物竞争。对于四种样本类型中的任何一种，使用DNA探针检测法均未获得假阳性结果。
