College of Agronomy & Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China.
Department of Plant Pathology, The Ohio State University, Columbus, OH 43202, USA.
Int J Mol Sci. 2019 Feb 21;20(4):933. doi: 10.3390/ijms20040933.
Wheat powdery mildew caused by f. sp. () is considered a major wheat leaf disease in the main wheat producing regions of the world. Although many resistant wheat cultivars to this disease have been developed, little is known about their resistance mechanisms. is a broad, effective resistance gene against powdery mildew in wheat line . The aim of this study was to investigate the resistance proteins after inoculation in wheat lines , , and . with was used as the resistant control, and without any effective genes was the susceptible control. Proteins were extracted from wheat leaves sampled 2, 4, 8, 12, and 24 h after inoculation, separated by two-dimensional electrophoresis, and stained with Coomassie brilliant blue G-250. The results showed that different proteins were upregulated and downregulated in three wheat cultivars at different time points. For the wheat cultivar , a total of 62 proteins were upregulated and 71 proteins were downregulated after inoculation. Among these, 46 upregulated proteins were identified by mass spectrometry analysis using the NCBI nr database of . The identified proteins were predicted to be associated with the defense response, photosynthesis, signal transduction, carbohydrate metabolism, energy pathway, protein turnover, and cell structure functions. It is inferred that the proteins are not only involved in defense response, but also other physiological and cellular processes to confer wheat resistance against . Therefore, the resistance products potentially mediate the immune response and coordinate other physiological and cellular processes during the resistance response to . The lipoxygenase, glucan exohydrolase, glucose adenylyltransferasesmall, phosphoribulokinase, and phosphoglucomutase are first reported to be involved in the interactions of wheat- at early stage. The further study of these proteins will deepen our understanding of their detailed functions and potentially develop more efficient disease control strategies.
小麦白粉病由 f. sp. ()引起，被认为是世界主要小麦产区的一种主要小麦叶片病害。尽管已经开发出许多对这种疾病具有抗性的小麦品种，但对其抗性机制知之甚少。 是一种广泛有效的抗小麦白粉病基因，存在于小麦品系 中。本研究旨在研究小麦品系 、 、 接种 后诱导的抗性蛋白。 用 作为抗性对照，不含任何有效 基因的 作为敏感对照。在接种后 2、4、8、12 和 24 小时从小麦叶片中提取蛋白质，通过二维电泳分离，并用考马斯亮蓝 G-250染色。结果表明，在三个小麦品种中，不同的蛋白质在不同的时间点被上调和下调。对于小麦品种 ，接种后共上调 62 种蛋白质，下调 71 种蛋白质。其中，使用 NCBI nr 数据库的质谱分析鉴定了 46 种上调蛋白。鉴定的蛋白质被预测与防御反应、光合作用、信号转导、碳水化合物代谢、能量途径、蛋白质周转和细胞结构功能有关。推断这些蛋白质不仅参与防御反应，还参与其他生理和细胞过程，赋予小麦对 的抗性。因此，抗性产物可能在对 的免疫反应中起作用，并在对 的抗性反应中协调其他生理和细胞过程。脂氧合酶、葡聚糖外切水解酶、葡萄糖腺苷酰转移酶小、磷酸核酮糖激酶和磷酸葡萄糖变位酶首次被报道参与小麦- 的早期相互作用。对这些蛋白质的进一步研究将加深我们对其详细功能的理解，并有可能开发出更有效的疾病控制策略。
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