Wheat, Peanut, and Other Field Crops Research Unit, USDA-ARS, Stillwater, OK, 74075, USA.
Plant and Soil Science Department, Oklahoma State University, Stillwater, OK, 74078, USA.
Theor Appl Genet. 2018 May;131(5):1145-1152. doi: 10.1007/s00122-018-3067-9. Epub 2018 Feb 16.
A new powdery mildew resistance gene, designated Pm59, was identified in Afghanistan wheat landrace PI 181356, and mapped in the terminal region of the long arm of chromosome 7A. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is an important foliar disease of wheat worldwide. In the Great Plains of the USA, Bgt isolates virulent to widely used powdery mildew resistance genes, such as Pm3a, were previously identified. The objectives of this study were to characterize the powdery mildew resistance gene in Afghanistan landrace PI 181356, which exhibited high resistance to Bgt isolates collected in southern Great Plains, and identify molecular markers for marker-assisted selection. An F population and F lines derived from a cross between PI 181356 and OK1059060-126135-3 were used in this study. Genetic analysis indicated that PI 181356 carries a single dominant gene, designated Pm59, in the terminal region of the long arm of chromosome 7A. Pm59 was mapped to an interval between sequence tag site (STS) markers Xmag1759 and Xmag1714 with genetic distances of 0.4 cM distal to Xmag1759 and 5.7 cM proximal to Xmag1714. Physical mapping suggested that Pm59 is in the distal bin 7AL 0.99-1.00. Pm59 is a novel powdery mildew resistance gene, and confers resistance to Bgt isolates collected from the Great Plains and the state of Montana. Therefore, Pm59 can be used to breed powdery mildew-resistant cultivars in these regions. Xmag1759 is ideal for marker-assisted selection of Pm59 in wheat breeding.
一个新的抗白粉病基因 Pm59 被鉴定为阿富汗小麦地方品种 PI 181356 中的一个基因，并被定位在 7A 染色体长臂的末端区域。白粉病是一种由禾本科布氏白粉菌引起的叶部病害，在全球范围内是小麦的一种重要病害。在美国大平原地区，以前曾鉴定出对广泛使用的白粉病抗性基因如 Pm3a 有毒性的 Bgt 分离物。本研究的目的是鉴定来自阿富汗地方品种 PI 181356 的白粉病抗性基因，该基因对大平原南部采集的 Bgt 分离物表现出高度抗性，并鉴定用于标记辅助选择的分子标记。本研究使用了来自 PI 181356 和 OK1059060-126135-3 杂交的 F 群体和 F 系。遗传分析表明，PI 181356 在 7A 染色体长臂的末端区域携带一个显性单基因，命名为 Pm59。Pm59 被定位在序列标签位点（STS）标记 Xmag1759 和 Xmag1714 之间的一个区间内，与 Xmag1759 的遗传距离为 0.4 cM，与 Xmag1714 的遗传距离为 5.7 cM。物理作图表明 Pm59 位于 7AL 0.99-1.00 的远端 bin 中。Pm59 是一个新的白粉病抗性基因，对来自大平原和蒙大拿州的 Bgt 分离物具有抗性。因此，Pm59 可用于这些地区培育抗白粉病的小麦品种。Xmag1759 是小麦育种中辅助选择 Pm59 的理想标记。
