Laddada Lilia, Jagla Krzysztof, Soler Cédric
GReD Laboratory, Clermont-Auvergne University, INSERM U1103, CNRS UMR6293, 63000 Clermont-Ferrand, France.
GReD Laboratory, Clermont-Auvergne University, INSERM U1103, CNRS UMR6293, 63000 Clermont-Ferrand, France
Biol Open. 2019 Mar 18;8(3):bio038760. doi: 10.1242/bio.038760.
Multiple tissue interactions take place during the development of the limb musculoskeletal system. While appendicular myogenesis has been extensively studied, development of connective tissue associated with muscles has received less attention. In the developing leg, tendon-like connective tissue arises from clusters of epithelial cells that invaginate into the leg cavity and then elongate to form internal tube-shape structures along which muscle precursors are distributed. Here we show that stripe-positive appendicular precursors of tendon-like connective tissue are set up among intersegmental leg joint cells expressing genes, and that Notch signaling is necessary and locally sufficient to trigger stripe expression. This study also finds that genes and are both required downstream of Notch to promote morphogenesis of tube-shaped internal tendons of the leg.
肢体肌肉骨骼系统发育过程中会发生多种组织相互作用。虽然附肢肌生成已得到广泛研究，但与肌肉相关的结缔组织发育受到的关注较少。在发育中的腿部，腱样结缔组织起源于上皮细胞簇，这些上皮细胞内陷进入腿部腔室，然后伸长形成内部管状结构，肌肉前体细胞沿此分布。在这里，我们表明，腱样结缔组织的条纹阳性附肢前体细胞在表达基因的节间腿部关节细胞中形成，并且Notch信号对于触发条纹表达是必要的且局部足够的。本研究还发现，基因和在Notch下游都是促进腿部管状内部肌腱形态发生所必需的。
