State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
Curr Genet. 2019 Aug;65(4):857-864. doi: 10.1007/s00294-019-00945-3. Epub 2019 Feb 22.
Single-stranded DNA (ssDNA) is widely generated during DNA metabolisms including DNA replication, repair and recombination and is susceptible to digestion by nucleases and secondary structure formation. It is vital for DNA metabolism and genome stability that ssDNA is protected and stabilized, which are performed by the major ssDNA-binding protein, and replication protein A (RPA) in these processes. In addition, RPA-coated ssDNA also serves as a protein-protein-binding platform for coordinating multiple events during DNA metabolisms. However, little is known about whether and how the formation of RPA-ssDNA platform is regulated. Here we highlight our recent study of a novel RPA-binding protein, Regulator of Ty1 transposition 105 (Rtt105) in Saccharomyces cerevisiae, which regulates the RPA-ssDNA platform assembly at replication forks. We propose that Rtt105 functions as an "RPA chaperone" during DNA replication, likely also promoting the assembly of RPA-ssDNA platform in other processes in which RPA plays a critical role.
单链 DNA（ssDNA）在包括 DNA 复制、修复和重组在内的 DNA 代谢过程中广泛产生，易受到核酸酶的消化和二级结构的形成。ssDNA 的保护和稳定对于 DNA 代谢和基因组稳定性至关重要，这是由主要的 ssDNA 结合蛋白和复制蛋白 A（RPA）在这些过程中完成的。此外，RPA 包裹的 ssDNA 也作为一个蛋白质-蛋白质结合平台，协调 DNA 代谢过程中的多个事件。然而，人们对 RPA-ssDNA 平台的形成是否受到调节以及如何受到调节知之甚少。在这里，我们强调了我们最近在酿酒酵母中对一种新型 RPA 结合蛋白 Regulator of Ty1 transposition 105（Rtt105）的研究，该蛋白调节复制叉处的 RPA-ssDNA 平台组装。我们提出，Rtt105 在 DNA 复制过程中充当“RPA 伴侣”，可能也促进了 RPA 在其他发挥关键作用的过程中组装 RPA-ssDNA 平台。
