对采自中国南方的圆尾鲎红树林鲎的毒素及毒性鉴定

Toxin and toxicity identification of mangrove horseshoe crab Carcinoscorpius rotundicauda collected from South China.

作者信息

Zheng Renjin, Guan Qiumei, Zheng Mi, Huang Zongxiu, Huang Hongnan, Fu Wusheng, Lin Shouer, Yang Yan

机构信息

Physical and Chemical Analysis Department, Fujian Center for Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou, 350001, PR China.

Fujian Medical University, Fuzhou, 350004, China.

出版信息

Toxicon. 2019 Apr 1;161:23-27. doi: 10.1016/j.toxicon.2019.02.002. Epub 2019 Feb 21.

DOI:10.1016/j.toxicon.2019.02.002

PMID:30796930

Abstract

The presence of paralytic shellfish poisoning (PSP), diarrhetic Shellfish Poisoning (DSP), tetrodotoxin (TTX) and its analogues (11-oxoTTX, 4.9-anhydro-11-oxoTTX, 4.9-anhydroTTX, 5-deoxyTTX, 5.11-dideoxyTTX, 5.6.11-trideoxyTTX and 4.9-anhydro-5.6.11-trideoxy TTX) were initially investigated in Carcinoscorpius rotundicauda collected from south China with Liquid chromatography-tandem mass spectrometry (LC-MS-MS) and mouse bioassay. The TTX toxicity was 10.8 ± 3.9 MU/g muscle, 6.3 ± 0.6 MU/g viscera and 6.3 ± 0.6 MU/g eggs in mean value. Merely dcGTX2 and dcSTX were detected in ten Specimens, ranging from 0.01 to 0.77 μg/g. Analyses suggested that these Carcinoscorpius rotundicauda contain TTX and its analogues as the major toxin and PSPs as the minor, respectively. Besides, no DSPs were found.

摘要

最初采用液相色谱 - 串联质谱法（LC-MS-MS）和小鼠生物测定法，对采自中国南方的圆尾鲎中是否存在麻痹性贝类毒素（PSP）、腹泻性贝类毒素（DSP）、河豚毒素（TTX）及其类似物（11-氧代TTX、4,9-脱水-11-氧代TTX、4,9-脱水TTX、5-脱氧TTX、5,11-二脱氧TTX、5,6,11-三脱氧TTX和4,9-脱水-5,6,11-三脱氧TTX）进行了调查。TTX毒性平均值在肌肉中为10.8±3.9 MU/g，在内脏中为6.3±0.6 MU/g，在卵中为6.3±0.6 MU/g。在十个样本中仅检测到dcGTX2和dcSTX，含量范围为0.01至0.77μg/g。分析表明，这些圆尾鲎分别以TTX及其类似物作为主要毒素，以PSP作为次要毒素。此外，未发现DSP。