Departmento de Biología de La Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México, 14080, México.
Departamento de Genómica Computacional, Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Ciudad de México, 14610, México.
Mol Cell Endocrinol. 2019 Apr 5;485:88-96. doi: 10.1016/j.mce.2019.02.010. Epub 2019 Feb 21.
The aim of this study was to analyze the effects of progesterone withdrawal on gene transcription in receptive endometrium by the administration of a single dose of 50 mg of the anti-progesterone receptor mifepristone (MFP) at the time of follicle rupture (FR). Six volunteer ovulatory women were studied, taking endometrial biopsies of three control and three MFP-treated women on days LH+2 (C-LH+2) and LH+7 (T-MFP), respectively. The biopsies were prepared for RNA isolation or histological and immunohistochemistry studies. The genomic data from 14 women (C-LH+7) were included as a historical control. The functional genomic analysis of the differentially expressed genes showed that MFP interfered negatively with the bio-functions decidualization of uterus and implantation of blastocyst and embryo. The results of this study confirm but also give new information on how MFP affects endometrial gene expression when administered at the time of FR and the dose used in emergency contraception.
本研究旨在通过在卵泡破裂（FR）时单次给予 50mg 抗孕激素受体米非司酮（MFP），分析孕激素撤退对接受性子宫内膜基因转录的影响。分别在 LH+2 天（C-LH+2）和 LH+7 天（T-MFP）对 6 名志愿者排卵妇女进行研究，对 3 名对照组和 3 名 MFP 治疗组妇女进行子宫内膜活检。将活检标本用于 RNA 分离或组织学和免疫组织化学研究。还将 14 名妇女（C-LH+7）的基因组数据作为历史对照纳入。差异表达基因的功能基因组分析表明，MFP 对子宫蜕膜化和胚泡及胚胎着床的生物功能产生负面影响。这项研究的结果不仅证实了 MFP 在 FR 时和紧急避孕中使用的剂量如何影响子宫内膜基因表达，而且还提供了新的信息。
