Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, Mexico.
Departamento de Genómica Computacional, Instituto Nacional de Medicina Genómica, Ciudad de México, Mexico.
Mol Cell Endocrinol. 2018 Nov 15;476:70-78. doi: 10.1016/j.mce.2018.04.010. Epub 2018 Apr 27.
In order to get further information on the effects of ulipristal acetate (UPA) upon the process of decidualization of endometrium, a functional analysis of the differentially expressed genes in endometrium (DEG) from UPA treated-versus control-cycles of normal ovulatory women was performed. A list of 1183 endometrial DEG, from a previously published study by our group, was submitted to gene ontology, gene enrichment and ingenuity pathway analyses (IPA). This functional analysis showed that decidualization was a biological process overrepresented. Gene set enrichment analysis identified LIF, PRL, IL15 and STAT3 among the most down-regulated genes within the JAK STAT canonical pathway. IPA showed that decidualization of uterus was a bio-function predicted as inhibited by UPA. The results demonstrated that this selective progesterone receptor modulator, when administered during the periovulatory phase of the menstrual cycle, may affect the molecular mechanisms leading to endometrial decidualization in response to progesterone during the period of maximum embryo receptivity.
为了进一步了解屈螺酮（UPA）对子宫内膜蜕膜化过程的影响，对正常排卵周期中接受 UPA 治疗与对照组的子宫内膜差异表达基因（DEG）进行了功能分析。我们的研究小组之前发表的一项研究中列出了 1183 个子宫内膜 DEG，提交给基因本体论、基因富集和 Ingenuity 通路分析（IPA）进行分析。该功能分析表明，蜕膜化是一个过表达的生物学过程。基因集富集分析确定 LIF、PRL、IL15 和 STAT3 是 JAK-STAT 经典通路中下调最明显的基因之一。IPA 显示，子宫蜕膜化是 UPA 预测的生物功能受到抑制。结果表明，这种选择性孕激素受体调节剂在月经周期的排卵前阶段给药时，可能会影响孕激素作用下导致子宫内膜蜕膜化的分子机制，从而影响胚胎最易受孕期的子宫内膜对孕激素的反应。
