基于氧化酶和 PtRu 纳米粒子的安培生物传感器作为人工过氧化物酶。
Amperometric biosensors based on oxidases and PtRu nanoparticles as artificial peroxidase.
机构信息
Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine.
Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine.
出版信息
Food Chem. 2019 Jul 1;285:213-220. doi: 10.1016/j.foodchem.2019.01.117. Epub 2019 Jan 24.
Catalytically active nanomaterials have several advantages over their natural analogues when used as artificial enzymes (nanozymes), namely, higher stability and lower cost. Nanozymes with metallic nanocomposites are promising catalysts for biosensing applications. The aim of the current research is to construct oxidase-based bioelectrodes for food analysis using nanozymes as peroxidase mimetics. Bimetallic PtRu nanoparticles (nPtRu) coupled with alcohol oxidase (AO) and methylamine oxidase (AMO) were chosen to construct amperometric biosensors (ABSs) for primary alcohols and methylamine (MA). Both ABSs show high sensitivities (336 A·M·m for the AO-ABS and 284 A·M·m for the AMO-ABS), broad linear ranges (25-200 µM ethanol and 20-600 µM MA) and satisfactory storage stabilities. Practical feasibility of the constructed ABSs was demonstrated on food samples. High correlation between contents of MA and ethanol in foods determined by the ABSs and reference methods was observed.
当用作人工酶（纳米酶）时，催化活性纳米材料相对于其天然类似物具有几个优势，即更高的稳定性和更低的成本。具有金属纳米复合材料的纳米酶是用于生物传感应用的有前途的催化剂。目前的研究旨在构建基于氧化酶的生物电极，用于使用纳米酶作为过氧化物酶模拟物进行食品分析。选择双金属 PtRu 纳米颗粒（nPtRu）与醇氧化酶（AO）和甲胺氧化酶（AMO）偶联，以构建用于伯醇和甲胺（MA）的电流型生物传感器（ABS）。这两种 ABS 都表现出高灵敏度（AO-ABS 为 336 A·M·m，AMO-ABS 为 284 A·M·m）、宽线性范围（25-200 µM 乙醇和 20-600 µM MA）和令人满意的存储稳定性。所构建的 ABS 在食品样品上的实际可行性得到了证明。通过 ABS 和参考方法测定的食品中 MA 和乙醇含量之间观察到高度相关性。
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